[Histonet] help ! paraffin section

[Mehmet Fatih BOZKURT]

In addition to Rene's comment,    to cut coagulated tissue (skin that have
new wound crust) and calcified tissue is difficult.

On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar <meghak <@t> g.clemson.edu> wrote:

> Hi All
> I am trying to section adult mouse intestine and skin using paraffin
> embedding. However, when i section, the tissue is torn although the rest of
> the paraffin looks perfect. Please suggest why this is happening. Also,
> sometimes the skin sections fall off the slides when I perform in situ
> hybridization. Any ideas how to prevent this?
> Please help! i am a beginner in histology and dont' know what to do!
> regards
> Megha
>
>
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-- 
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-173/237