[Histonet] tissue highlighting for visibility

[Vanessa Perez]

We use microwave processing and we add hematoxylin to the absolute and eosin to the isopropyl....this also helps in keeping techs from accidently using isopropyl as absolute or vice-versa...  when grosser cant find tissue in the container we put a drop of eosin and swirl and filter to try and find any material in the formalin container.


Vanessa Perez Garcia
Histology Supervisor
Pathology Reference Lab
210-892-3746
210-892-3732
vperez <@t> pathreflab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of MaryK Mendell
Sent: Wednesday, August 08, 2012 5:48 AM
To: Lee & Peggy Wenk; contact <@t> histocare.com; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] tissue highlighting for visibility

ditto on this.  I also have very tiny specimens and this works wonderful, but use the smallest of drop

Kate Mendell
Histopathology/Lab Manager

HOWARD S. GOLDBERG, M.D., INC
990 Paradise Road
Swampscott, MA  01907
TEL:  781.595.0151
FAX:  781.592.6780
kmendell <@t> goldbergmd.net
www.cosmesticdermcenter.com
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From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpwenk <@t> sbcglobal.net]
Sent: Wednesday, August 08, 2012 5:33 AM
To: contact <@t> histocare.com; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] tissue highlighting for visibility

Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

The opinions expressed are mine, and do not reflect those of Beaumont Hospital.

-----Original Message-----
From: contact <@t> histocare.com
Sent: Tuesday, August 07, 2012 6:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue highlighting for visibility



Hello all,

Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort.

I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing.  What do some of you guys do?

www.HistoCare.com
Histology Staffing

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