[Histonet] tissue highlighting for visibility

Lee & Peggy Wenk lpwenk <@t> sbcglobal.net
Wed Aug 8 04:33:45 CDT 2012

Drop of hematoxylin on the tissue, when put on the paper in the grossing 
area. Use a syringe. Only a SMALL drop. Too much means there's extra blue 
all over the paper, making it hard to see the blue tissue.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

The opinions expressed are mine, and do not reflect those of Beaumont 

-----Original Message----- 
From: contact <@t> histocare.com
Sent: Tuesday, August 07, 2012 6:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue highlighting for visibility

Hello all,

Earlier today I had a VERY tiny sample from the esophogus. When I say it was 
tiny, it looked to be only a few microns in thickness. It was inside of, you 
guessed it,
a teabag! :) But that wasn't the problem, as it was appropriate in this case 
to be put in a teabag because of the size. When I pulled it out of the 
cassette, I had to go over it very carefully to even find it. It's sad that 
I know of a not insignificant number of people who wouldn't have taken the 
time to find it and most likely have dispositioned it as not surviving 
processing or no tissue found, but that is another issue. I'm sure the 
patient would appreciate the extra effort.

I know of a few techniques to make tissue, and specifically tiny or fatty 
tissue, more easily visible in cases like these. For example, I've seen 
using a different colored wax or putting eosin in the alcohol during 
processing.  What do some of you guys do?

Histology Staffing

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list