[Histonet] help ! paraffin section

[Megha Kumar]

Hi All
I am trying to section adult mouse intestine and skin using paraffin
embedding. However, when i section, the tissue is torn although the rest of
the paraffin looks perfect. Please suggest why this is happening. Also,
sometimes the skin sections fall off the slides when I perform in situ
hybridization. Any ideas how to prevent this?
Please help! i am a beginner in histology and dont' know what to do!
regards
Megha


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