[Histonet] counterstain for Alcian Blue (ph2.5)

[Amos Brooks]

Hi,
     We use Neutral Red in 1% solution. (Not being in the lab right now I
think it is made up in 1% acetic acid, but I could be wrong.) It is much
faster than NFR (nucear fast red). It is about one minute to stain as
opposed to the 5 to 15 if the NFR solution is old. It is easy as heck to
make up and neutral red has a much longer shelf life too. The only
difference I have noted is that the stain is a slightly darker (rusty) red
than the *really* light pink of NFR.

Amos

On Wed, Aug 1, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 4
> Date: Wed, 1 Aug 2012 12:50:14 -0400
> From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
> Subject: [Histonet] counterstain for Alcian Blue (ph2.5)
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <DCFD9E6A390E294AAF3A2561CD32E5C417A90481 <@t> ckhamail1.ckha.on.ca>
> Content-Type: text/plain;       charset="us-ascii"
>
> Can you please let me know what options for counterstain there are other
> than Nuclear Fast Red or a supplier who sells the prepared solution
>
>
>
> Diana
>