[Histonet] ACTH Antibody

[Tiana Baskin]

Hi I am working with the DAKO ACTH clone 02A3 (concentrate) and I am
getting a lot of background staining. I have tried to decreasing the
incubation time of the primary and I have tried to increase the dilution
however I am starting to loose signal and I still have the background
and non-specific staining. I was wondering if anyone has had this
experience and whether or not anyone is happy with and/or using the
Cellmarque polyclonal ACTH?

 

Thanks :-)

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