[Histonet] size of cut/transfer method affect staining?

[Smith, Matthew A.]

Hi,

  I am attempting to do dual staining with 2 antibodies.  One works fine but I am having trouble getting consistent staining with the second.  I recently noticed previous user was requesting 3uM microtomy sections on tape, then going TPC, xylenes/etoh, AR,etc.  I have been using 4um floated sections and can just not see the same staining using same everything else.  Does anyone have thoughts about this?  I was thinking perhaps the TPC/tape leaves the cells more permeable or affects AR efficiency.  I am currently going to test side-by-side...just trying to get a general opinion on the matter as immunohistochemistry is not my area of proficiency.



Matthew A. Smith, PhD MSPH
Postdoctoral Fellow, Clinical Investigations
Laboratory of Eric Haura, MD
tel:  813-745-6193 | email:  Matthew.Smith <@t> moffitt.org<mailto:Matthew.Smith <@t> moffitt.org>


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