[Histonet] size of cut/transfer method affect staining?
Smith, Matthew A.
Matthew.Smith <@t> moffitt.org
Wed Apr 25 14:18:09 CDT 2012
I am attempting to do dual staining with 2 antibodies. One works fine but I am having trouble getting consistent staining with the second. I recently noticed previous user was requesting 3uM microtomy sections on tape, then going TPC, xylenes/etoh, AR,etc. I have been using 4um floated sections and can just not see the same staining using same everything else. Does anyone have thoughts about this? I was thinking perhaps the TPC/tape leaves the cells more permeable or affects AR efficiency. I am currently going to test side-by-side...just trying to get a general opinion on the matter as immunohistochemistry is not my area of proficiency.
Matthew A. Smith, PhD MSPH
Postdoctoral Fellow, Clinical Investigations
Laboratory of Eric Haura, MD
tel: 813-745-6193 | email: Matthew.Smith <@t> moffitt.org<mailto:Matthew.Smith <@t> moffitt.org>
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