[Histonet] Re: Histonet Digest, Vol 101, Issue 27

[Amos Brooks]

Hi,
     Strange issue with your Ki-67. I don't know what would do that unless
it was non-specific background. Is it nuclear or cutoplasmic?
     If you are working in mice or other animals you could try labeling
them with BRDu then detecting it with an anti BRDu antibody. Humans don't
like being injected with BRDu then being sacrifices but lawyers do. Also
Cleaved Caspase 3 works nicely and is probably more specific to apoptosis
than Tunel which also picks up necrosis.

Have a great weekend,
Amos


On Fri, Apr 20, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 9
> Date: Thu, 19 Apr 2012 15:07:43 -0500
> From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
> Subject: [Histonet] proliferation and apoptosis
> To: "histonet-request <@t> lists.utsouthwestern.edu"
>        <histonet-request <@t> lists.utsouthwestern.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
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> C1BA93040C6B9A4A8D84878F93FEC36A0AB7D0E9F1 <@t> CMHEXCC01MBX.childrensmemorial.org
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> Hi histonetters,
>
> I am looking for the good markers to detect (separately) proliferation and
> apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic
> bodies as well as proliferated cells; and the tunnel assay shows both
> apoptotic body and proliferation.
>
> Any suggestions for the FFPE tissue and the HRP protocol are appreciated.
>
> Thanks in advance,
> Naira
>