Re: [Histonet] RE: GMS on Toenail

Thu Apr 19 16:24:49 CDT 2012

Are using any adhesive on the slide or a charged slide?  We used to dip slides in a water/elmer glue solution and allow to dry.  Then place a section on it and it seemed to work...

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----- Reply message -----
From: "Kienitz, Kari" <kkienitz <@t> orclinic.com>
Date: Thu, Apr 19, 2012 12:33 pm
Subject: [Histonet] RE: GMS on Toenail
To: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>

After cutting, try putting the slide into a coplin jar of formalin.  Introduce to heat for about 30 minutes or so, remove and let air dry before staining.

Kari Kienitz HT, (ASCP)
Histology Laboratory
Portland Gastroenterology
The Oregon Clinic
1111 NE 99th Ave
Portland, OR  97220
503.935.8311
kkienitz <@t> orclinic.com
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [Allison_Scott <@t> hchd.tmc.edu]
Sent: Thursday, April 19, 2012 12:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GMS on Toenail

Hello to all in histoland.  We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine.  Any suggestions on how to keep the section on the slide during the staining procedure.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas

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