[Histonet] Batch Controls

Debra Siena DSiena <@t> statlab.com
Thu Apr 19 09:46:45 CDT 2012

After spending 7 1/2 years as a technical specialist with Ventana, I would also like to say that putting the control on the bottom of the slide and the patient at the top is just added insurance that the patient will receive the bulk of the reagents vs. the control which will help ensure against false negatives should there be a staining issue.  Just my 2 cents worth.  thanks

  Debbie Siena, HT(ASCP)QIHC
StatLab Medical Products
Technical Support Manager
407 Interchange Street | McKinney, TX 75071
t: 800.442.3573 ext. 229 | f: 972.436.1369
dsiena <@t> statlab.com | www.statlab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Thursday, April 19, 2012 9:20 AM
To: histonet
Subject: RE: [Histonet] Batch Controls

I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one.  
Many of us use some kind of automated immunostainer where there is no "gaurantee" that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked.  Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control.
For this reason alone, one should think hard about using batch controls.
Just My Opinion,
Glen Dawson  BS, HT(ASCP) & QIHC
Histology Technical Specialist
Janesville, WI 		 	   		  _______________________________________________
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