[Histonet] Re: IHC in porcine tissues

[Galina Deyneko]

Dear Elizabeth and Colleagues
I found in Abcam two ABs clone MAC387 : ab 22506 (with your reference) and ab80708.
Also if somebody interested I found new polymer from Biocare: mouse-on-pharma HRP polymer that is intended to work in bovine, porcine,ovine tissues. Not expensive- 231$.
I am going to try.
Thank you all for your responses.


Galina Deyneko
Novartis, Cambridge, MA
617-782-1675 home
617-871-7613 w

--- On Mon, 4/16/12, Elizabeth Chlipala <liz <@t> premierlab.com> wrote:


From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] Re: IHC in porcine tissues
To: "'Galina Deyneko'" <galinadeyneko <@t> yahoo.com>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, April 16, 2012, 3:10 PM


Galina

Try MAC387 that is a macrophage/monocyte marker that cross reacts in porcine.  We use the one from abcam.  We have found that some of the anti-mouse polymer reagents cross react to porcine so we use a rabbit anti-mouse from Dako E0464 as a secondary and then an envision rabbit or strept avidin and that works much better.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Galina Deyneko
Sent: Monday, April 16, 2012 1:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: IHC in porcine tissues



Dear Colleagues
What  secondary antibody or polymer would be better to use for mouse primary antibody on FFPE  porcine tissues?Also, please share your experience regarding macrophages detection in porcine arteries. I found couple in Abcam and Serotec (only for frozen), but your opinions is very valuable.
Thank you

Galina Deyneko
Novartis, Cambridge, MA
617-782-1675 home
617-871-7613 w

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