[Histonet] Citrate Buffer pH2.0

Goins, Tresa TGoins <@t> mt.gov
Tue Apr 17 12:00:43 CDT 2012


I haven't made stock solution recently, but I believe the starting pH of solution prior to adding NaOH is less than 2, so this should work.

Citrate pH 3.0     use at 10 mM

      For a 10 x stock solution [100 mM]
      10.5 g citric acid monohydrate [MW 210.14] in 370 ml water
      adjust the pH to 3.0 with concentrated NaOH
      add 2.5 ml Tween 20
      adjust final volume to 500 ml


Tresa Goins
Montana Veterinary Diagnostic Lab
Bozeman, Montana 59718


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Neil Macintyre
Sent: Tuesday, April 17, 2012 10:26 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Citrate Buffer pH2.0

Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen retrieval as this pH has been recommended by the company supplying  AM3K macrophage antibody and I can't find this in any of the usual IHC buffer protocols.

Neil MacIntyre CSci FIBMS
Laboratory Manager
Veterinary Pathology Unit
The Royal(Dick)School of Veterinary Studies The University Of Edinburgh Easter Bush Campus Midlothian
EH25 9RG

0131 650 6403/8802

The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336.

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