[Histonet] Methyl green going away...

[Tony Henwood (SCHN)]

You could try dehydrating in acetone (very quick).

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Alonso Martinez-Canabal
Sent: Tuesday, 17 April 2012 1:27 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Methyl green going away...

  Hi 

my boss requires me to counterstain some DAB immunohistochemistry with methyl green. I use for that purpose the methyl  green in aquos solution 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate and the methyl green becomes extremely faint with the alcohols. My protocol requires long periods of alcoholic dehydration very graded. Is there any idea on how to save the methyl green. Can I use an alcoholic solution?
Thank you very much. 

Alonso 

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