[Histonet] Re: Histonet Digest, Vol 101, Issue 21

[Madeleine Huey]

> Message: 1
> Date: Mon, 16 Apr 2012 11:26:47 -0400
> From: "Alonso Martinez-Canabal" <alonso.martinezcanabal <@t> utoronto.ca>
> Subject: [Histonet] Methyl green going away...
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca>
> Content-Type: text/plain;       charset="US-ASCII"
>
>  Hi
>
> my boss requires me to counterstain some DAB immunohistochemistry with
> methyl green. I use for that purpose the methyl  green in aquos solution
> 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate
> and the methyl green becomes extremely faint with the alcohols. My protocol
> requires long periods of alcoholic dehydration very graded. Is there any
> idea on how to save the methyl green. Can I use an alcoholic solution?
> Thank you very much.
>
> Alonso

Alonso,

Another alternative is; Drying the slide in the oven @~ 60c  for 10
min after the methly green counterstain.

Madeleine Huey BS, HTL/QIHC (ASCP)
Supervisor - Pathology (IPOX & Histology)
madeleinehuey <@t> elcaminohospital.org