[Histonet] Methyl green going away...

Alonso Martinez-Canabal alonso.martinezcanabal <@t> utoronto.ca
Mon Apr 16 10:26:47 CDT 2012


my boss requires me to counterstain some DAB immunohistochemistry with
methyl green. I use for that purpose the methyl  green in aquos solution
0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate
and the methyl green becomes extremely faint with the alcohols. My protocol
requires long periods of alcoholic dehydration very graded. Is there any
idea on how to save the methyl green. Can I use an alcoholic solution?
Thank you very much. 


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