[Histonet] Powdered egg white for endogenous biotin blocking

Jonathan Cremer Jonathan.Cremer <@t> med.kuleuven.be
Thu Apr 12 07:14:09 CDT 2012

Does anyone know if the powdered egg white from Sigma (E0500) still contains active streptavidin? I would like to use it to block endogenous biotin instead of going through the hassle of obtaining fresh eggs, but I've read that avidin is inactivated by heat (pasteurization, in this case).

Also, if anyone already uses powdered egg white for this purpose, what concentration? Will 5% do?

Many thanks,


Jonathan Cremer
Laboratory Technician
TARGID - KU Leuven

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