[Histonet] Loss of Matrigel material during staining

[Carrie Schray]

My experience with this is that the processing schedule is too long
and probably too much heat. I had some skin that was grown in a well
plate from human cells processed on a biopsy run, they cut fantastic
then the gel rolled onto itself during staining. Once we reduced the
processing schedule and took heat away on the processing reagents it
was much better.
And, try to use super sticky slides, such as those you would use with
bone samples.

Carrie L. Schray
Univ. of Michigan Medical School
Unit for Laboratory Animal Medicine
Ann Arbor, Michigan


On 4/5/12, Amy Porter <portera <@t> msu.edu> wrote:
> To all:  I have a client that we are attempting to stain H & E / as well as
> immuno on FFPE mouse skin with matrigel plugs.  These things section like a
> dream and then when we attempt to stain only the matrigel part of the sample
> is falling off.  Does anyone have any tricks with this stuff they would be
> willing to share?  We are using charged slides and cutting at 5-6 microns
> per the client.  Thanks -
>
>
>
> Amy S. Porter, HT(ASCP) QIHC
>
> Michigan State University
>
> Investigative HistoPathology Laboratory
>
> William S. Spielman, Ph.D. - Director
>
> Patricia K. Senagore, M.D. - Consulting Pathologist
>
> Department of Physiology / Human Pathology
>
> Biomedical Physical Sciences Building
>
> 567 Wilson Road - Room 2133
>
> East Lansing, MI  48824-3320
>
> Phone:  517-884-5026
>
> Fax:  517-432-1368
>
> portera <@t> msu.edu
>
> www.humanpathology.msu.edu
>
>
>
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