[Histonet] Re:SAVE MAsson's in BONE info
Vicki Kalscheur
kalschev <@t> svm.vetmed.wisc.edu
Thu Apr 5 12:15:51 CDT 2012
----- Original Message -----
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, April 05, 2012 12:05 PM
Subject: Histonet Digest, Vol 101, Issue 4
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> Today's Topics:
>
> 1. RE: Slippery Floor due to paraffin (Sebree Linda A)
> 2. New Position Alert - IHC Field Tech Atlanta (Matt Ward)
> 3. Glassware washing (Oneil, Beth Ann)
> 4. Re: Massons Trichrome on decalcified bone (gayle callis)
> 5. Automatic knife sharpener (Maxim Peshkov)
> 6. Grossing rules (Amber McKenzie)
> 7. EM tissue processors Q's (Morken, Timothy)
> 8. Specimen Identity Procedure (Scott, Allison D)
> 9. Re: EM tissue processors Q's (Jan Shivers)
> 10. Bone (Trichrome) (Lawrence Allen)
> 11. Region III final reminder (Shirley A. Powell)
> 12. Leica Bond IHC Platform (Wellen, Terrence D. :LPH Lab)
> 13. (no subject) (Khaire Dai)
> 14. please don't sent e-mail (mervatawad <@t> aol.com)
> 15. Re: Massons Trichrome on decalcified bone (Sara Landschoot)
> 16. RE: Leica Bond IHC Platform (Sue Hunter)
> 17. Re: Re: Massons Trichrome on decalcified bone (Jack Ratliff)
> 18. RE: Leica Bond IHC Platform (Rathborne, Toni)
> 19. RE: Leica Bond IHC Platform (Sue Hunter)
> 20. Commercial clearing and bluing solutions in Europe
> (Jackie O'Connor)
> 21. JOB OPENING (Jeffrey Howery)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 4 Apr 2012 12:21:24 -0500
> From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
> Subject: RE: [Histonet] Slippery Floor due to paraffin
> To: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F0B9FD4B28C80E43996FFD4408B198AE01EFF354 <@t> UWHC-MAIL3.uwhis.hosp.wisc.edu>
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Peel off film here...works well.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott,
> Allison D
> Sent: Wednesday, April 04, 2012 11:50 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Slippery Floor due to paraffin
>
> Hello to all in histoland. What are histology labs doing to combat the
> slipperiness of the floor due to paraffin. Are you using rugs, peel
> away films ? Any help would be greatly appreciated.
>
>
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas 77026
>
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>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 4 Apr 2012 13:31:10 -0400
> From: Matt Ward <mw <@t> personifysearch.com>
> Subject: [Histonet] New Position Alert - IHC Field Tech Atlanta
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <33b696097ef326f96a51a0f52df63358 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Good Afternoon Histonet,
>
>
>
> We have had a global leader in IHC and Histology open a field based IHC
> support opportunity covering the Southeast Region. The ideal location
> would
> be to be based in Atlanta and the position is open due to promotion.
>
>
>
> If you or anyone you may know has a strong background in IHC and is
> looking
> to break out of the lab into a field role please contact me directly to
> learn more.
>
>
>
> The position offers a Base Salary + Bonus + Full Benefits (Car Allowance,
> Corporate Credit Card, Cell Phone, Laptop, Home Office, Full Health,
> 401k).
>
>
>
> Regards,
>
>
>
>
>
> Matt Ward
>
> *Account Executive*
>
> *Personify*
>
> 5020 Weston Parkway Suite 315
>
> Cary NC 27513
>
> (Tel) 800.875.6188 direct ext 103
>
> (Fax) 919.460.0642
>
> www.personifysearch.com
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 4 Apr 2012 18:08:14 +0000
> From: "Oneil, Beth Ann" <oneilb <@t> wvuhealthcare.com>
> Subject: [Histonet] Glassware washing
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <3CEB8EBCF9C7A648B9694B5696462A71EFE0 <@t> NT-EXMB2.wvuh.wvuhs.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Our laboratory does about half of it's daily special stains manually which
> means we go through a lot of Copeland jars. We currently wash everything
> by hand using liquinox with a splash of bleach in the water. We rinse
> with tap water. My question is how do other labs address the CAP issue of
> testing their glassware for detergent removal. When I worked in the
> chemistry lab, we didn't go through a lot of glassware so we were able to
> take a monthly bin of dirty glassware to sterile processing for cleaning,
> and then just check one representative piece of glassware from the batch
> for detergent. I can't figure out how my lab can check our glassware when
> there is so much, we wash it all throughout the day. Thank you for any
> help.
>
> Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
> Histology Supervisor/Technical Specialist
> West Virginia University Hospitals
> 304-293-7629 (office)
> 304-293-6014 (lab)
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 4 Apr 2012 12:13:26 -0600
> From: "gayle callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] Re: Massons Trichrome on decalcified bone
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000a01cd128e$a30c7110$e9255330$@bresnan.net>
> Content-Type: text/plain; charset="US-ASCII"
>
> Dear Sara,
>
>
>
> You wrote:
>
> I work at an Orthopaedic research lab and I have been having some trouble
> getting our trichromes to work on our bone. I have tried a masson's,
> gomori,
> and goldner's and in all cases the bone stained red.
>
>
>
> Our bone is arrive to our lab frozen, we fix them in formalin, embed in
> paraffin, and decal with EDTA. I also post fix with Bouins before
> staining.
>
>
>
> Techs that worked in this lab before me were also getting the same red
> staining of bone.
>
>
>
> Is there something during processing that could cause this reaction? We
> fix
> in formalin, dehydrate through graded alcohols, and clear in xylene.
>
>
>
> ****************************************************************************
> ****************************
>
> I doubt your processing has any effect on Mass Tri staining. However,
> incomplete decalcification can but I suspect it may be the staining
> protocol
> itself. Using a simple weight loss/weight gain decalcification end point
> test with EDTA is a good idea, and can be used for acid decalcification
> endpoint testing IF you are not presently using an endpoint test.
>
>
>
> Years ago, I visited the AFIP bone lab, and acquired a Massons Trichrome
> protocol which worked much better than the standard Massons Trichrome
> found
> in most textbooks. It never failed to work well with our decalcified bone
> work although we did use acid decalcification. This is NOT a kit
> method.
> All reagents are made up in house, and post mordant heating Bouins is NOT
> done in a microwave. We preferred to let the sections sit in Boiuns
> overnight at RT, or heat in a water bath. We never used a dry heat,
> incubator type oven where one gets uneven heating in the chamber.
>
>
>
> There is more to doing Massons Trichrome on decalcified bone other than
> just
> following the recipe from a textbook. One of the best discussions on
> understanding the chemistry/theory of trichrome staining is found in
> Sheehan
> and Hrapchak, Theory and Practice of Histotechnology.
>
>
>
> The AFIP method has some different staining times, plus how to remove the
> biebrich scarlet/acid fuchsin from collagen BEFORE going into aniline
> blue,
> a much more controlled staining method. It also has a modified Weigerts
> Iron hematoxylin that is superior, a bit more concentrated since Iron
> hematoxylin tends to be removed by the acidic staining solutions. I will
> be happy to send these methods to you privately.
>
>
>
> Gayle M. Callis
>
> HTL/HT/MT(ASCP)
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 4 Apr 2012 23:20:15 +0300
> From: Maxim Peshkov <Maxim_71 <@t> mail.ru>
> Subject: [Histonet] Automatic knife sharpener
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <1182039392.20120404232015 <@t> mail.ru>
> Content-Type: text/plain; charset=windows-1251
>
> Dear Histonetters!
> Can anybody to advise, what type automatic knife sharpener
> we can buy in Europe (Germany) instead Leica SP9000,
> which now is unavailable for sale? What vendors?
> We do not like a Shandon "Autosharp 5" type sharpener.
> Maxim Peshkov
> Russia,
> Taganrog.
> mailto:Maxim_71 <@t> mail.ru
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 4 Apr 2012 19:26:06 +0000
> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] Grossing rules
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <5A33C952BB67F4468AF1F36D739212BC11631D48 <@t> JERRY.Gia.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Are there any guidelines on how to do GI grossing? At what size to you
> bisect? Do you call 5 or more pieces multiple or do you count all the
> pieces? How many categories are there: 1, 2, 3, several, multiple,
> etc...At what size is it considered a fragment and what size is a polyp?
> Thanks!
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 4 Apr 2012 12:26:19 -0700
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: [Histonet] EM tissue processors Q's
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <8D7C2D242DBD45498006B21122072BF8A4DDF412 <@t> MCINFRWEM003.ucsfmedicalcenter.org>
>
> Content-Type: text/plain; charset=us-ascii
>
> Hi, we're looking around at different automated TEM tissue processors. We
> have an RMC 5160 but it has had a lot of problems so we are considering
> something else. I've looked online at Leica TP and Lynx II for routine
> processing. We may get to MW processing later, but the price is higher
> than authorized right now.
>
> Any suggestions or comments?
>
> Thanks for any info!
>
>
> Tim Morken
> Supervisor, Electron Microscopy
> Department of Pathology
> UC San Francisco Medical Center
> 505 Parnassus Ave, Box 1656
> Room S570
> San Francisco, CA 94115
>
> (415) 353-2673 (ph)
> (415) 514-3403 (fax)
> tim.morken <@t> ucsfmedctr.org
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 4 Apr 2012 19:57:46 +0000
> From: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>
> Subject: [Histonet] Specimen Identity Procedure
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <FA607DC3D1ED7C46A9546820A3EB877F1870A0 <@t> LBMSG02.hchd.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello again to all in histoland. Does anyone have a procedure for
> describing the system for maintaining the identity of every specimen
> through processing and block and slide preparation. This is on the CAP
> checklist (ANP.21050). Any help will be greatly appreciated.
>
>
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas
>
> CONFIDENTIALITY NOTICE:
> If you have received this e-mail in error, please immediately notify the
> sender by return e-mail and delete this e-mail and any attachments from
> your computer system.
>
> To the extent the information in this e-mail and any attachments contain
> protected health information as defined by the Health Insurance
> Portability
> and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
> 164; or Chapter 181, Texas Health and Safety Code, it is confidential
> and/or
> privileged. This e-mail may also be confidential and/or privileged under
> Texas law. The e-mail is for the use of only the individual or entity
> named
> above. If you are not the intended recipient, or any authorized
> representative of the intended recipient, you are hereby notified that any
> review, dissemination or copying of this e-mail and its attachments is
> strictly prohibited.
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 4 Apr 2012 15:05:50 -0500
> From: Jan Shivers <shive003 <@t> umn.edu>
> Subject: Re: [Histonet] EM tissue processors Q's
> To: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CAEoC1q0wWhYhqxqCMgJ85FYykD9SN2w-g-7+x8LD2WOEn2=ysg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> We use the Leica TP for our samples and endorse it.
>
> Jan Shivers
> IHC/Histo/EM Section Head
> Veterinary Diagnostic Lab
> UMN College of Veterinary Medicine
> St. Paul, MN
>
> On Wed, Apr 4, 2012 at 2:26 PM, Morken, Timothy <
> Timothy.Morken <@t> ucsfmedctr.org> wrote:
>
>> Hi, we're looking around at different automated TEM tissue processors. We
>> have an RMC 5160 but it has had a lot of problems so we are considering
>> something else. I've looked online at Leica TP and Lynx II for routine
>> processing. We may get to MW processing later, but the price is higher
>> than
>> authorized right now.
>>
>> Any suggestions or comments?
>>
>> Thanks for any info!
>>
>>
>> Tim Morken
>> Supervisor, Electron Microscopy
>> Department of Pathology
>> UC San Francisco Medical Center
>> 505 Parnassus Ave, Box 1656
>> Room S570
>> San Francisco, CA 94115
>>
>> (415) 353-2673 (ph)
>> (415) 514-3403 (fax)
>> tim.morken <@t> ucsfmedctr.org
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 4 Apr 2012 17:03:44 -0400
> From: Lawrence Allen <Histolaw69 <@t> aol.com>
> Subject: [Histonet] Bone (Trichrome)
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <EC19B851-7E3E-4DD2-B276-F5B3741C2B5B <@t> aol.com>
> Content-Type: text/plain; charset=us-ascii
>
>
> Why not sent in 10 % formalin. Freezing it may change the morphology of
> the bone to fixation after thawing. I would give that a try. After all,
> you are running 3 different stains at one time in order.
>
> Lawrence S Allen
> Lead Histotechnologist
> Dorm VA Medical Center
> Columbia, SC
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 4 Apr 2012 17:54:42 -0400
> From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
> Subject: [Histonet] Region III final reminder
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9BF995BC0E47744E9673A41486E24EE24B330F1C3D <@t> MERCERMAIL.MercerU.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Histotechs,
>
> This email is to remind you of the Georgia Society for Histotechnology
> hosting Region III meeting at Callaway Gardens, Pine Mountain GA April
> 13-15th,. This is next week friends. We will NOT be in the conference
> center as last year, and that is a GOOD thing. We will be in the ballroom
> area where we will have more space. We have far exceeded our original
> block of rooms, extended the deadline and now if the Mountain Creek Inn
> has any available, they will allow the GSH room rate. Please use the GSH
> Group # # 78K711 to get the discounted room rate of $109. Make
> reservations now at 1-800-225-5292.
>
> If you have not registered for the meeting please do so. PLEASE
> REGISTER NOW AS "AWAITING FUNDS" if you are waiting approval for the
> meeting go ahead and register for your workshops, luncheon, dinner and
> note on the form that you are awaiting funds below the total line. When
> approved, then send the funds, or if time does not allow, pay on site.
> But please register as soon as possible, don't wait, workshops are filling
> up fast.
>
> We also will have Histotalk's Dave Kemler at the meeting to interview
> some of our attendees as well.
>
> Also make plans to attend the Carriage and Horses Dinner on Saturday
> night for great food, great friends and good entertainment. The deadline
> for reservations for the dinner is April 9th, that is next Monday. Please
> make sure you fill out your registration form completely and email the
> form to Anne Taylor, GSH Treasurer to confirm your workshops as awaiting
> funds and then pay at the registration desk on site since time is short.
>
> If you have questions or concerns please contact Mike Ayers at
> lmayers <@t> charter.net<mailto:lmayers <@t> charter.net>, Wanda Simons at
> Wandrous <@t> att.net<mailto:Wandrous <@t> att.net> or myself at
> powell_sa <@t> mercer.edu<mailto:powell_sa <@t> mercer.edu>.
>
> We are excited to have 29 vendors exhibiting at our meeting and more
> signing up as we approach the meeting.
>
> BioCare ~Sponsor of new IHC Award
>
> BioGenex
>
> B/R Instruments
>
> Cancer Diagnostics
>
> Cell Marque
>
> Choice Medical
>
> Clarient~ sponsoring dinner at Dagher's
>
> Dako
>
> EMS
>
> Epitomics
>
> General Data
>
> IMEB
>
> Lab Storage
>
> Leica Microsystems ~ sponsoring dinner at Dagher's
>
> Leica Biosystems
>
> Mopec
>
> PolyScientific R&D - sponsor of new award "TBA"
>
> Sakura - Sponsor of GSH Histotechnologist of the Year Award
>
> Stat Lab
>
> Southeast Pathology
>
> Thermo Fisher -
>
> TBS
>
> ScyTek
>
> StatLab
>
> Ventana
>
>
>
> Those Not attending but supporting GSH are:
>
> Anatech - Unmanned table
>
> CL Sturkey - Door prizes
>
> LABSCO - Sponsoring a break
>
> Newcomer - Sponsoring a Break and provided totes
>
> Come Experience ____________
> Histotechnology - Southern Style
>
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 4 Apr 2012 17:06:04 -0700
> From: "Wellen, Terrence D. :LPH Lab" <TWELLEN <@t> LHS.ORG>
> Subject: [Histonet] Leica Bond IHC Platform
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <5F46C87AD649864F9FA8273E8E31507A1607E0 <@t> SWM2006.LHSNT.LEGACYHS>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Does anyone have any experience with this product?
>
>
> Terrence Wellen HT(ASCP)
> Legacy Good Samaritan Hospital
> Portland, OR
>
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 4 Apr 2012 20:46:34 -0700 (PDT)
> From: Khaire Dai <khairedai <@t> yahoo.com>
> Subject: [Histonet] (no subject)
> To: histonet <@t> lists.utsouthwestern.edu, kgrobert <@t> rci.rutgers.edu
> Message-ID:
> <1333597594.50037.YahooMailMobile <@t> web113010.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> <a
> href="http://gearsnow.com/wp-content/plugins/extended-comment-options/fjgvkd.html">
> http://gearsnow.com/wp-content/plugins/extended-comment-options/fjgvkd.html</a>
>
> ------------------------------
>
> Message: 14
> Date: Thu, 5 Apr 2012 06:09:51 -0400 (EDT)
> From: mervatawad <@t> aol.com
> Subject: [Histonet] please don't sent e-mail
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8CEE12B60147DDD-13BC-3A0F <@t> webmail-d157.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
> ------------------------------
>
> Message: 15
> Date: Thu, 5 Apr 2012 08:20:20 -0500
> From: Sara Landschoot <sllandsc <@t> gmail.com>
> Subject: [Histonet] Re: Massons Trichrome on decalcified bone
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CAObz69XUXvT4Af4W2QbaoTgp6=knNjYcbpkEL32+7pO7Xvk0tA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Gayle,
>
> Thanks so much for your response on Histonet. I have been getting several
> different answers that I have been trying to test.
>
> I can give you more details on how we decal our bone here. We deal with
> entire sheep or primate spines which are grossed while frozen into slabs.
> The slabs are about 5-6mm thick. We take an initial xray to get a starting
> point then we decal the slabs in EDTA (after formalin fixation). Xrays are
> taken throughout the decal process to check for the endpoint.
>
> As for post fixing in Bouins, I was using the water bath method (60
> degrees) for about an hour.
>
> If you don't mind sending me the AFIP methods so I can try then out on my
> sections I would greatly appreciate it. My email is sllandsc <@t> gmail.com.
>
> Again thank you for your help and I look forward to hearing from you.
>
> Sara Landschoot
>
>
> ------------------------------
>
> Message: 16
> Date: Thu, 5 Apr 2012 13:23:57 +0000
> From: Sue Hunter <SHUNTER <@t> beaumont.edu>
> Subject: [Histonet] RE: Leica Bond IHC Platform
> To: "Wellen, Terrence D. :LPH Lab" <TWELLEN <@t> LHS.ORG>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B493B3E4EF638A41875845CEDF938B44014CCC93 <@t> EXMail04.ms.beaumont.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> We have a Bond Max in my lab and love it! It has been very dependable and
> easy to use . We also have three Ventana Ultras and two Lab Visions. We
> originally got the Bond for EBV and Kappa/Lambda in-situ but also do our
> hormone receptors and a few other immunos on it. Our pathologists felt
> the signal obtained with the DAB on the in-situ slides was superior to the
> NBT of the Ventana slides. The one draw back that Ventana will talk about
> is that you have three racks of ten slides - each rack is independent of
> the others, but not the continual load of the Ultras. You also cannot mix
> pretreatments on each rack because of timing/heating issues. But we have
> not found either of these two issues to be a problem for us. The Bond III
> is supposed to be even faster than the Bond Max but haven't looked at
> that.
> Sue
>
> Sue Hunter
> Supervisor, Advanced Diagnostics
> Beaumont Health Systems
> Royal Oak MI 48073
> 248-898-5146
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Wellen,
> Terrence D. :LPH Lab
> Sent: Wednesday, April 04, 2012 8:06 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Leica Bond IHC Platform
>
>
> Does anyone have any experience with this product?
>
>
> Terrence Wellen HT(ASCP)
> Legacy Good Samaritan Hospital
> Portland, OR
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> This message has been scanned and no issues discovered.
> To report this email as SPAM, please forward it to spam <@t> websense.com.
>
>
>
> ------------------------------
>
> Message: 17
> Date: Thu, 5 Apr 2012 10:06:32 -0400
> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
> Subject: Re: [Histonet] Re: Massons Trichrome on decalcified bone
> To: Sara Landschoot <sllandsc <@t> gmail.com>
> Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU0-SMTP263328D88DD847AA7FC7CFBAE330 <@t> phx.gbl>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Sara!
>
> Might I quickly ask why you decalcify instead of processing and embedding
> undecalcified using methyl methacrylate resin/plastic? I routinely process
> these type of specimens into MMA and cut either 5 micron sections without
> metal present or thick/ground sections polished to 30-40 microns if a
> metallic device is present.
>
> These same specimens that have been processed and embedded into plastic
> and cut at 5 microns can be deplasticized prior to staining with H&E,
> Goldner's trichrome, VonKossa-MacNeal's tetrachrome, Safranin O-Fast
> Green, Sirius Red-Fast Green, etc. If these specimens contain a large
> metal implant, the sections are stained undeplasticized with Sanderson's
> Rapid Bone Stain & Van Gieson picrofuchsin.
>
> Of course of you don't have these capabilities or equipment to process
> these specimens into resin, decalcification is your only option, but I
> would personally use 5% or 10% Formic acid instead of the lengthy EDTA
> process. I would also use methyl salicylate to replace the xylenes steps
> so that you can avoid making the bone too brittle and difficult to cut.
>
> Feel free to message me back if you need further explanation or if you
> would even like to discuss privately by phone. I would also be happy to
> forward you stained images of these stains, previously listed and from
> this same specimen type you are working with, that has been resin
> embedded. Also, I have trained people all over the world to process
> undecalcified bone (any size) into resin/plastic and cut at 5 microns if
> this is an option for you!
>
> Best Regards,
>
> Jack
>
> Jack Ratliff
> Hard Tissue Histologist
> Chairman, Hard Tissue Committee - National Society for Histotechnology
>
> On Apr 5, 2012, at 9:20 AM, Sara Landschoot <sllandsc <@t> gmail.com> wrote:
>
>> Hi Gayle,
>>
>> Thanks so much for your response on Histonet. I have been getting several
>> different answers that I have been trying to test.
>>
>> I can give you more details on how we decal our bone here. We deal with
>> entire sheep or primate spines which are grossed while frozen into slabs.
>> The slabs are about 5-6mm thick. We take an initial xray to get a
>> starting
>> point then we decal the slabs in EDTA (after formalin fixation). Xrays
>> are
>> taken throughout the decal process to check for the endpoint.
>>
>> As for post fixing in Bouins, I was using the water bath method (60
>> degrees) for about an hour.
>>
>> If you don't mind sending me the AFIP methods so I can try then out on my
>> sections I would greatly appreciate it. My email is sllandsc <@t> gmail.com.
>>
>> Again thank you for your help and I look forward to hearing from you.
>>
>> Sara Landschoot
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Thu, 5 Apr 2012 14:50:52 +0000
> From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
> Subject: [Histonet] RE: Leica Bond IHC Platform
> To: "'Sue Hunter'" <SHUNTER <@t> beaumont.edu>, "Wellen, Terrence D. :LPH
> Lab" <TWELLEN <@t> LHS.ORG>, "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <3AD061FE740D464FAC7BF6B5CFB7570711FBB66D <@t> SMCMAIL01.somerset-healthcare.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We have the Bond III. No complaints. I'm not sure what to compare to
> regarding speed, but we can do 4 runs a day if necessary. This does not
> include an overnight run, which we do as needed.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sue Hunter
> Sent: Thursday, April 05, 2012 9:24 AM
> To: Wellen, Terrence D. :LPH Lab; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Leica Bond IHC Platform
>
> We have a Bond Max in my lab and love it! It has been very dependable and
> easy to use . We also have three Ventana Ultras and two Lab Visions. We
> originally got the Bond for EBV and Kappa/Lambda in-situ but also do our
> hormone receptors and a few other immunos on it. Our pathologists felt
> the signal obtained with the DAB on the in-situ slides was superior to the
> NBT of the Ventana slides. The one draw back that Ventana will talk about
> is that you have three racks of ten slides - each rack is independent of
> the others, but not the continual load of the Ultras. You also cannot mix
> pretreatments on each rack because of timing/heating issues. But we have
> not found either of these two issues to be a problem for us. The Bond III
> is supposed to be even faster than the Bond Max but haven't looked at
> that.
> Sue
>
> Sue Hunter
> Supervisor, Advanced Diagnostics
> Beaumont Health Systems
> Royal Oak MI 48073
> 248-898-5146
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Wellen,
> Terrence D. :LPH Lab
> Sent: Wednesday, April 04, 2012 8:06 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Leica Bond IHC Platform
>
>
> Does anyone have any experience with this product?
>
>
> Terrence Wellen HT(ASCP)
> Legacy Good Samaritan Hospital
> Portland, OR
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> This message has been scanned and no issues discovered.
> To report this email as SPAM, please forward it to spam <@t> websense.com.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical Center
> and are intended only for the addressee. The information contained in
> this
> message is confidential and may contain privileged, confidential,
> proprietary and/or trade secret information entitled to protection and/or
> exemption from disclosure under applicable law. Unauthorized forwarding,
> printing, copying, distribution, or use of such information is strictly
> prohibited and may be unlawful. If you are not the addressee, please
> promptly delete this message and notify the sender of the delivery error
> by e-mail or you may call Somerset Medical Center's computer Help Desk
> at 908-685-2200, ext. 4050.
>
> Be sure to visit Somerset Medical Center's Web site -
> www.somersetmedicalcenter.com - for the most up-to-date news,
> event listings, health information and more.
>
>
>
> ------------------------------
>
> Message: 19
> Date: Thu, 5 Apr 2012 15:12:20 +0000
> From: Sue Hunter <SHUNTER <@t> beaumont.edu>
> Subject: [Histonet] RE: Leica Bond IHC Platform
> To: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>, "Wellen,
> Terrence D. :LPH Lab" <TWELLEN <@t> LHS.ORG>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B493B3E4EF638A41875845CEDF938B44014CCD49 <@t> EXMail04.ms.beaumont.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> I've only been told that the Bond III could do a run in, say 3 hours,
> where the Bond Max might be done in 4.(don't take these times as true -
> just as an example). We love the ability to do a delayed start so the
> slides are done when we come in the next morning - especially the in-situ
> slides that take longer than the immunos.
> sue
>
> -----Original Message-----
> From: Rathborne, Toni [mailto:trathborne <@t> somerset-healthcare.com]
> Sent: Thursday, April 05, 2012 10:51 AM
> To: Sue Hunter; Wellen, Terrence D. :LPH Lab;
> histonet <@t> lists.utsouthwestern.edu
> Subject: RE: Leica Bond IHC Platform
>
> We have the Bond III. No complaints. I'm not sure what to compare to
> regarding speed, but we can do 4 runs a day if necessary. This does not
> include an overnight run, which we do as needed.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sue Hunter
> Sent: Thursday, April 05, 2012 9:24 AM
> To: Wellen, Terrence D. :LPH Lab; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Leica Bond IHC Platform
>
> We have a Bond Max in my lab and love it! It has been very dependable and
> easy to use . We also have three Ventana Ultras and two Lab Visions. We
> originally got the Bond for EBV and Kappa/Lambda in-situ but also do our
> hormone receptors and a few other immunos on it. Our pathologists felt
> the signal obtained with the DAB on the in-situ slides was superior to the
> NBT of the Ventana slides. The one draw back that Ventana will talk about
> is that you have three racks of ten slides - each rack is independent of
> the others, but not the continual load of the Ultras. You also cannot mix
> pretreatments on each rack because of timing/heating issues. But we have
> not found either of these two issues to be a problem for us. The Bond III
> is supposed to be even faster than the Bond Max but haven't looked at
> that.
> Sue
>
> Sue Hunter
> Supervisor, Advanced Diagnostics
> Beaumont Health Systems
> Royal Oak MI 48073
> 248-898-5146
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Wellen,
> Terrence D. :LPH Lab
> Sent: Wednesday, April 04, 2012 8:06 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Leica Bond IHC Platform
>
>
> Does anyone have any experience with this product?
>
>
> Terrence Wellen HT(ASCP)
> Legacy Good Samaritan Hospital
> Portland, OR
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> This message has been scanned and no issues discovered.
> To report this email as SPAM, please forward it to spam <@t> websense.com.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical Center
> and are intended only for the addressee. The information contained in
> this message is confidential and may contain privileged, confidential,
> proprietary and/or trade secret information entitled to protection and/or
> exemption from disclosure under applicable law. Unauthorized forwarding,
> printing, copying, distribution, or use of such information is strictly
> prohibited and may be unlawful. If you are not the addressee, please
> promptly delete this message and notify the sender of the delivery error
> by e-mail or you may call Somerset Medical Center's computer Help Desk at
> 908-685-2200, ext. 4050.
>
> Be sure to visit Somerset Medical Center's Web site -
> www.somersetmedicalcenter.com - for the most up-to-date news, event
> listings, health information and more.
> This message has been scanned and no issues discovered.
> To report this email as SPAM, please forward it to spam <@t> websense.com.
>
>
>
> ------------------------------
>
> Message: 20
> Date: Thu, 5 Apr 2012 11:18:53 -0400 (EDT)
> From: "Jackie O'Connor" <b427297 <@t> aol.com>
> Subject: [Histonet] Commercial clearing and bluing solutions in Europe
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8CEE1568C202EBD-1A70-18B07 <@t> webmail-m011.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> I am looking for vendors in Germany who can provide commercially prepared,
> RTU H+E clarifier and bluing reagents. Any advice? Leica does not
> distribute these products in Germany, I've been told.
> Thanks,
> Jackie O'
>
>
> ------------------------------
>
> Message: 21
> Date: Thu, 5 Apr 2012 15:36:46 +0000
> From: Jeffrey Howery <Jeffrey.Howery <@t> jcl.com>
> Subject: [Histonet] JOB OPENING
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <40985CBB26DB2F4D8FBC273E835992E79625 <@t> JCLNSEXMAIL02.jclnt.jcl.com>
> Content-Type: text/plain; charset="us-ascii"
>
> I am just putting this out there but we have an opening here at John C
> Lincoln Deer Valley Phoenix, Arizona.
> If anyone might be interested please contact me. I can help fill you in on
> what we are looking for.
> Thanks Jeff
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 101, Issue 4
> ****************************************
>
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