[Histonet] AFB by IHC
Rcartun <@t> harthosp.org
Tue Apr 3 15:45:09 CDT 2012
Yes, you can do IHC for mycobacteria; however, there are issues with specificity depending on the primary antibody that you use. I have been using a polyclonal antibody from Dako (no longer available) for many years and although it is very sensitive for identifying mycobacteria, it does cross-react with other bacteria. I have not found a monoclonal antibody (yet) that works on formalin-fixed, paraffin-embedded tissue. In addition, your IHC assay has to be exquisitely clean in order to trust the rare immunoreactivity that may be present.
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> Amy Self <ASelf <@t> georgetownhospitalsystem.org> 4/3/2012 11:48 AM >>>
My pathologist wants an AFB stain done by IHC. Is this stain/procedure even doable and if so where?
Thanks in advance for your help, Amy
Georgetown Hospital System
The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet