[Histonet] AFB by IHC

Richard Cartun Rcartun <@t> harthosp.org
Tue Apr 3 15:45:09 CDT 2012

Yes, you can do IHC for mycobacteria; however, there are issues with specificity depending on the primary antibody that you use.  I have been using a polyclonal antibody from Dako (no longer available) for many years and although it is very sensitive for identifying mycobacteria, it does cross-react with other bacteria.  I have not found a monoclonal antibody (yet) that works on formalin-fixed, paraffin-embedded tissue.  In addition, your IHC assay has to be exquisitely clean in order to trust the rare immunoreactivity that may be present.


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> Amy Self <ASelf <@t> georgetownhospitalsystem.org> 4/3/2012 11:48 AM >>>
My pathologist wants an AFB stain done by IHC.  Is this stain/procedure even doable and if so where?

Thanks in advance for your help,  Amy

Amy Self
Georgetown Hospital System
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