[Histonet] AFB by IHC

[Richard Cartun]

Yes, you can do IHC for mycobacteria; however, there are issues with specificity depending on the primary antibody that you use.  I have been using a polyclonal antibody from Dako (no longer available) for many years and although it is very sensitive for identifying mycobacteria, it does cross-react with other bacteria.  I have not found a monoclonal antibody (yet) that works on formalin-fixed, paraffin-embedded tissue.  In addition, your IHC assay has to be exquisitely clean in order to trust the rare immunoreactivity that may be present.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Amy Self <ASelf <@t> georgetownhospitalsystem.org> 4/3/2012 11:48 AM >>>
My pathologist wants an AFB stain done by IHC.  Is this stain/procedure even doable and if so where?

Thanks in advance for your help,  Amy



Amy Self
Georgetown Hospital System
843-527-7179
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