[Histonet] Contract to Hire Opportunity for Histotech in CA
Erik Dokken
Erik.Dokken <@t> onassignment.com
Thu Sep 29 19:13:21 CDT 2011
On Assignment Healthcare has a contract to hire opportunity for a Histotech on the SF Peninsula.
This position will start ASAP and is full time.
Employees will be eligible for benefits upon completion of 30 days of work.
Competitive pay and referral bonus available.
If you would like to hear more contact On Assignment Healthcare today at 650-866-3386.
Erik Dokken
Area Manager - No. CA, WA, and TX
On Assignment, Inc. Local Allied Healthcare
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, September 20, 2011 10:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 94, Issue 23
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Today's Topics:
1. Gary Steinke is out of the office (Gary_Steinke <@t> vwr.com)
2. Ken Marissael is out of the office (Ken_Marissael <@t> vwr.com)
3. Histotech needed (Joyce Cline)
4. Diane Tokugawa/CA/KAIPERM is out of the office.
(Diane.Tokugawa <@t> kp.org)
5. How to store paraffin blocks ? (Itai Moshe)
6. SV: [Histonet] How to store paraffin blocks ?
(Erikstad, Gudrun Hovstein)
7. Verification System (Ann Angelo)
8. Re: How to store paraffin blocks ? (Rene J Buesa)
9. RE: How to store paraffin blocks ? (Sherwood, Margaret)
10. Tissue handling at embedding (Morken, Timothy)
11. Uni-trieve, antigen retrieval for immunofluorescence
(Andrea Marion)
----------------------------------------------------------------------
Message: 1
Date: Mon, 19 Sep 2011 14:02:34 -0400
From: Gary_Steinke <@t> vwr.com
Subject: [Histonet] Gary Steinke is out of the office
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF26A39913.598AB0D9-ON85257910.00631CD0-85257910.00631CD0 <@t> vwr.com>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting 09/19/2011 and will not return until 09/26/2011.
I will be out of the office from September 19th and returning on September 26th. I will out of town attending a business meeting and will have very limited access to email and voice mail. If you need assistance immediately, please contact Customer Service at 877-881-1192. Take care and have a great day.
------------------------------
Message: 2
Date: Mon, 19 Sep 2011 14:03:30 -0400
From: Ken_Marissael <@t> vwr.com
Subject: [Histonet] Ken Marissael is out of the office
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF2BE77FA1.14F1DA64-ON85257910.006332BE-85257910.006332BE <@t> vwr.com>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting 09/19/2011 and will not return until 09/23/2011.
I will be away on 9/16 and return 9/27. I will have limited e-mail and cell phone access, but will try to get back to you as quickly as possible. While I am away, please contact VWR Healthcare Customer Service at 877-881-1192.
------------------------------
Message: 3
Date: Mon, 19 Sep 2011 14:19:16 -0400
From: Joyce Cline <Joyce.Cline <@t> meritushealth.com>
Subject: [Histonet] Histotech needed
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2197D76184F4408CFEBD95C2828268A9D2154DB3 <@t> WCHSXCHCM.wchsys.org>
Content-Type: text/plain; charset="iso-8859-1"
Full time position, M-F varying shifts 6:00am to 6:00pm, rotating bench assignments, IHC, special stains and autopsies. No Saturdays or week end call.
We are near Wash D.C., Baltimore and other site seeing areas.
Go to Meritushealth.com HR department for job listings.
Joyce Cline, H. T. (ASCP)
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cline <@t> meritushealth.com<mailto:joyce.cline <@t> meritushealth.com>
________________________________
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Message: 4
Date: Mon, 19 Sep 2011 16:25:31 -0700
From: Diane.Tokugawa <@t> kp.org
Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF5A9B342E.3040601B-ON88257910.0080ADF2-88257910.0080ADF3 <@t> kp.org>
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I will be out of the office starting 09/19/2011 and will not return until 09/21/2011.
Note: For Cytology issues, please call Molly at 8-421-5487, Eric at
8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario
at 8-421-4961, general histology lab 8-421- 5408 or Wanda at 8-421-5426.
------------------------------
Message: 5
Date: Tue, 20 Sep 2011 09:23:04 +0300
From: Itai Moshe <itai.moshe <@t> mail.huji.ac.il>
Subject: [Histonet] How to store paraffin blocks ?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CACp9T1rgTxaVu+EA2QzRgavckD1wtper3qU3mOpEvmWh9DZcrA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Histonet's,
What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?
The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?
Thank's
Itai Moshe
------------------------------
Message: 6
Date: Tue, 20 Sep 2011 08:55:28 +0200
From: "Erikstad, Gudrun Hovstein" <Gudrun.Erikstad <@t> stolav.no>
Subject: SV: [Histonet] How to store paraffin blocks ?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D3D3E3C2F409474DA3E8CAD7C5B635A601699904 <@t> TREXCHVS01.stolav.helsemn.no>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
We store paraffin blocks and stained slides at room temperature in rooms without windows, so there is no sunlight.
We store blocks and slides "forever". We have paraffin blocks from before 1930.
"Room temperature" in Norway is usually about 20 degrees Celcius.
Some stained slides will fade with time (silver techniques, immuno), but the routine HE usually last for many, many years.
After we excanged Xylene with a substitute (Tissue-Clear) four years ago, we had some "bleeding" and fading of the HE-stains, but after taking more care changing the Xylene substitute in the clearing step more often this is no more a problem.
The paraffin blocks keep very well. We frequently do section and stain as well as do molecular techniques (PCR, In situ) on material from the 1970'ies and before, and it's remarkable how well the formalin fixed, paraffin embedded tissue keep.
I would not keep the blocks in a fridge, as you say, the humidity in the fridge is quite high.
The time to cool a block from room temperature to suitable for sectioning is only about two minutes.
Now, because Norway has a relatively cold climate, the air humidity is also quite low compared to warmer climates, even if it rains here ALL the time... ;-) Heat and high humidity might affect your blocks.
Gudrun H. Erikstad, Trondheim, Norway
-----Opprinnelig melding-----
Fra: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] P? vegne av Itai Moshe
Sendt: 20. september 2011 08:23
Til: histonet <@t> lists.utsouthwestern.edu
Emne: [Histonet] How to store paraffin blocks ?
Hi Histonet's,
What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?
The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?
Thank's
Itai Moshe
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 20 Sep 2011 10:09:41 -0400 (EDT)
From: Ann Angelo <thisisann <@t> aol.com>
Subject: [Histonet] Verification System
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CE45B6132A22E2-7D8-725E9 <@t> webmail-d167.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
I am looking for a verification system which does not require the data entry to be performed prior to cutting (ruling out Vantage and Cerebro). I have looked at Thermo's slidemate but can't get them to move fast enough.
Does anyone know of another verification system that I may be able to try which does not require the data entry to be performed prior to microtomy? Thanks, Ann
------------------------------
Message: 8
Date: Tue, 20 Sep 2011 07:19:47 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] How to store paraffin blocks ?
To: histonet <@t> lists.utsouthwestern.edu, Itai Moshe
<itai.moshe <@t> mail.huji.ac.il>
Message-ID:
<1316528387.5562.YahooMailClassic <@t> web65706.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The usual procedure is to store at room temperature. It is assumed that the room is air conditioned, otherwise if the temp is above 80?F some blocks may stick together, although that is not a real problem.
If at 4?C you will need a refrigerated room or a series of refrigerators, which is an additional and unnecessary expenditure.
We use to store the blocks for 9 years, unless it is a special case (used in teaching) and those are stored forever.
Supposedly you use the slides for some procedure and those cut as "extras" are kept along with the originals stained in the same files.
If you are referring to sections for IHC or other procedure, the epitopes will be oxidized by the oxygen in the air in less than 2 weeks, so they can either be stored in a fridge, or covered with melted paraffin, or stored in mineral oil.
Ren? J.
--- On Tue, 9/20/11, Itai Moshe <itai.moshe <@t> mail.huji.ac.il> wrote:
From: Itai Moshe <itai.moshe <@t> mail.huji.ac.il>
Subject: [Histonet] How to store paraffin blocks ?
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 AM
Hi Histonet's,
What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?
The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?
Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Tue, 20 Sep 2011 10:58:52 -0400
From: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] How to store paraffin blocks ?
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>, "Itai Moshe"
<itai.moshe <@t> mail.huji.ac.il>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9708DB5A01 <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="iso-8859-1"
We are a research group, but had paraffin blocks stored at room temperature for
20+ years. We finally made the decision to just keep 2 years in the lab
20+ and
store 5 years off-site. We trashed the rest.
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 20, 2011 10:20 AM
To: histonet <@t> lists.utsouthwestern.edu; Itai Moshe
Subject: Re: [Histonet] How to store paraffin blocks ?
The usual procedure is to store at room temperature. It is assumed that the room is air conditioned, otherwise if the temp is above 80?F some blocks may stick together, although that is not a real problem.
If at 4?C you will need a refrigerated room or a series of refrigerators, which is an additional and unnecessary expenditure.
We use to store the blocks for 9 years, unless it is a special case (used in
teaching) and those are stored forever.
Supposedly you use the slides for some procedure and those cut as "extras" are kept along with the originals stained in the same files.
If you are referring to sections for IHC or other procedure, the epitopes will be oxidized by the oxygen in the air in less than 2 weeks, so they can either be stored in a fridge, or covered with melted paraffin, or stored in mineral oil.
Ren? J.
--- On Tue, 9/20/11, Itai Moshe <itai.moshe <@t> mail.huji.ac.il> wrote:
From: Itai Moshe <itai.moshe <@t> mail.huji.ac.il>
Subject: [Histonet] How to store paraffin blocks ?
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 AM
Hi Histonet's,
What is the recommended way to store paraffin blocks for a long period ?
Room temperature, or 4C ?
The advantage in storing at 4C is that the blocks are always ready for sectioning.
Is there a risk when storing at 4C that the humidity in the fridge will cause damage to the paraffin blocks and the tissue ?
After sectioning, How do you store the slides, and for how long ?
Thank's
Itai Moshe
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
------------------------------
Message: 10
Date: Tue, 20 Sep 2011 09:44:26 -0700
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] Tissue handling at embedding
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8D7C2D242DBD45498006B21122072BF85E2AEE21 <@t> MCINFRWEM003.ucsfmedicalcenter.org>
Content-Type: text/plain; charset=us-ascii
What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost?
And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)?
Thanks for all info!
Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA
415.514.6042 (office)
415.885.7409 Fax
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
------------------------------
Message: 11
Date: Tue, 20 Sep 2011 11:59:19 -0500
From: "Andrea Marion" <amario3 <@t> uic.edu>
Subject: [Histonet] Uni-trieve, antigen retrieval for
immunofluorescence
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6623612296b9078e4db4eccef8f88c74.squirrel <@t> webmail.uic.edu>
Content-Type: text/plain;charset=iso-8859-1
Hello all,
Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported to be a universal antigen retrieval solution that can be used at lower temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):
http://innvx.com/unitrievepage.html
The company claims that the reagent is a universal retrieval solution for all antibodies and tissues (which is silly of course - how could they know?). Does anyone have any experience with the product?
I am interested because I see that increased heat during antigen retrieval causes greater tissue autofluorescence during immunofluorescence stainings. My current protocol is to use 20 minutes at 90-95 C on a hot plate using sodium citrate buffer. Does anyone else either Uni-trieve or a different reagent/protocol for immunofluorescence stainings?
Thanks,
Andrea
Andrea Marion
Graduate Student
University of Illinois at Chicago
------------------------------
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