[Histonet] Background in FFPE glandular tissue sections during IHC

[mesruh turkekul]

Dear Histonetters,


When I do IHC on FFPE tissue sections. Most of time there is background due
to trapping of IgG/detection reagents probably in the connective tissue
fibers, mucins or other secretions of the glandular tissue. Especially
prostate and mammary gland. Any tips to get rid of that kind of background?
Do you know any treatment  to block mucins or connective tissue fibers from
 nonspecifically and electrostatically attracting IgGs and detection
reagents?

Regards,
Mesruh Turkekul
mskcc.org