[Histonet] IHC H.P. Negative Control

[Sheila Fonner]

CAP guidelines clearly state that you must run a negative control for each patient tissue.  However, if you are running a large amount of a particular antibody, you may validate your procedure to run only one positive control.  However, it is just as easy to run a positive control for each patient if you put the control on the same slide as the patient tissue.

I don't see any way around the negatives though. We run a negative control on each and every block.

Sheila


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh
Sent: Tuesday, September 27, 2011 1:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC H.P. Negative Control

Hi Histonetters,

We would like to start to run up to 30 H.Pylori on our Benchmark daily.  Do GI labs that run H.P. on ALL stomach biopsies run a negative control for each patient tissue for H.P.?  I am just curious, as we have a 24 hour TAT, but the Benchmark only holds 30 slides.  What's everyone doing?

Thanks!

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