[Histonet] Kappa/ Lambda ISH Probes on BOND III
Jackie Smith
roosmith1 <@t> hotmail.com
Mon Sep 26 19:13:04 CDT 2011
Histonetters...
Would anyone performing ISH probes for Kappa & Lambda on the BOND III be willing to share their protocol for B+ fixed, decaled, paraffin-embedded bone marrow core sections. I have tried a variety of different combinations and times with ER1, ER2, Enzyme & incubation, yielding only minimal, suboptimal, quality staining.
Any input is greatly appreciated!
Kindest Regards, Eric SmithCelligent DiagnosticsCharlotte, NC
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