[Histonet] Kappa/ Lambda ISH Probes on BOND III

Jackie Smith roosmith1 <@t> hotmail.com
Mon Sep 26 19:13:04 CDT 2011


Histonetters... 
Would anyone performing ISH probes for Kappa & Lambda on the BOND III be willing to share their protocol for B+ fixed, decaled, paraffin-embedded bone marrow core sections.  I have tried a variety of different combinations and times with ER1, ER2, Enzyme & incubation, yielding only minimal, suboptimal, quality staining.  
Any input is greatly appreciated! 
Kindest Regards,  Eric SmithCelligent DiagnosticsCharlotte, NC 		 	   		  


More information about the Histonet mailing list