AW: [Histonet] nonstructured proteins

[Gudrun Lang]

After a short look into wikipedia I assume, that these proteins can also
bind formaldehyde as long as there are aminogroups of lysin and arginine
reachable. Crosslinking would be possible, if bound methylgroups come near
enough to peptidbinding. If this occurs intra- or intermolecular, who knows?

Do you build your own antibodies?

Gudrun
 

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Perry,
Margaret
Gesendet: Montag, 26. September 2011 19:22
An: ihcrg <@t> googlegroups.com; histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] nonstructured proteins

I am posting this again to see if anyone has worked with nonstructured
proteins.  Do they form the same crosslinks when fixed with formalin?  Would
antigen retrieval be of any use?  Do they react with IHC the same as
structured proteins?


Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet