[Histonet] Block age/labile epitopes - UPDATE

[gayle callis]

Liz, 

Pdf has been sent via private email.   

Gayle Callis 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Monday, September 26, 2011 9:17 AM
To: 'Richard Cartun'; Histonet; Andrea T. Hooper; Rene J Buesa
Subject: RE: [Histonet] Block age/labile epitopes - UPDATE

Does anyone have a pdf of this that they are willing to share.

Thanks

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO
Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, September 26, 2011 9:00 AM
To: Histonet; Andrea T. Hooper; Rene J Buesa
Subject: Re: [Histonet] Block age/labile epitopes - UPDATE

An interesting article on this subject came out earlier this year from
Stephen Hewitt's work group at the NCI.  Their work shows that the loss of
antigenicity in archival formalin-fixed, paraffin-embedded tissue sections
is due to the presence of water, both endogenously (poor
processing) and exogenously (humidity).  I've listed the reference
below:

Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation of
archival formalin-fixed, paraffin-embedded tissue sections.  J Histochem
Cytochem 59:356-365, 2011.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 9/25/2011 11:36 AM >>>
Andrea:
Under separate cover I am sending an article I wrote on the subject of
epitope oxidation including some  of theose easier to oxidize.
As to when a block is too old to be useless for IHC, that depends on the
epitope and the way the blocks have been stored.
I have been able to use 25 years old blocks and  the only thing you have to
do is to go deep into the block to reach an area where the epitopes have not
been oxidized yet.
René J.

--- On Sat, 9/24/11, Andrea T. Hooper <andreahooper <@t> rocketmail.com>
wrote:


From: Andrea T. Hooper <andreahooper <@t> rocketmail.com>
Subject: [Histonet] Block age/labile epitopes
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Saturday, September 24, 2011, 11:44 PM


Just curious as to at what shelf-life people consider their blocks too "old"
to rely on for IHC data? Along those lines I am wondering about labile
antigens ... does anyone know how fast epitopes may "disappear"
from blocks and what are some good examples of labile epitopes/antigens?
As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
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