[Histonet] AO-860 Sliding Microtome + OCT Embedded

[Caroline Bass]

I've tried something very similar with fresh brain tissue. I snap froze the tissue and then mounted it on the dry ice stage with little problems. But when I cut the tissue it thawed instantly on the knife and turned into a mess, even the thicker sections. You need to maintain the sections at a cold temperature outside the block as well, so a freezer room could help. 

Of course this technique works perfectly with formalin fixed sections.

> Message: 5
> Date: Fri, 23 Sep 2011 15:35:36 -0400
> From: Francis OBrien <francisobrien2007 <@t> gmail.com>
> Subject: Re: [Histonet] AO-860 Sliding Microtome + OCT Embedded
> 	Tissue?
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> 	histonet <@t> lists.utsouthwestern.edu
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> 	<CAKmFSPfJPkRpO-yxPOwgrZg=5MrhHO5GN5hc2gNmKBpXvdc3gA <@t> mail.gmail.com>
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> 
> I would be using the freezing stage with dry ice.