[Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?

[Caroline Bass]

Hi Francis,

I don't know how thick your sections will be, but in general the AO-860 (fantastic microtome by the way) is not designed to cut fresh tissue. Certainly the relatively thick sections brain sections (50-200 micron) won't stand up very well in my experience. How thick are you trying to go and what's the tissue. It might make a difference.

Caroline


> On Sep 23, 2011, at 1:05 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
> 
> Message: 4
> Date: Fri, 23 Sep 2011 12:03:03 -0400
> From: Francis OBrien <francisobrien2007 <@t> gmail.com>
> Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<CAKmFSPfpqDo1eBMg_BCfsKvaJZpLCeVyTDkq2HVp_DZKkr8ZQw <@t> mail.gmail.com>
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> 
> Hello,
> 
> I am a newbie to this type of work. We have flash frozen tissue in OCT
> (Optimal Cutting Temperature) compound for some unique experiments
> that we need to carry out. We want to use a AO-860 sliding microtome
> to cut large slabs of OCT embedded tissue. Does anyone have any advice
> on how this would work reproducibly and reliably on the freezing
> stage.
> 
> Best,
> 
> Francis
>