[Histonet]
Tissue tracking at embedding RE: Histonet Digest, Vol 94, Issue 24
Steve McClain
SteveM <@t> mcclainlab.com
Thu Sep 22 11:04:23 CDT 2011
Tim
You may consider asking Dr. Phil LeBoit at UCSF Dermpath and see what he
says.
I wrote about floaters a few weeks ago and this subject is related in my
view.
McClain Labs uses a combination of
1) manual line drawings on the papers reqs to indicate # of pieces
and approximate size;
a. we write on reqs # of pieces per block
2) record # of pieces per block in computer;
a. printed embedding log with # of pieces per block
3) photographs of tissue before and after slicing. (see attached
images- not available to other Histonet readers)
The embedders have access to all three forms of data- redundancy is
being a good thing.
By convention, we limit # of pieces to 5 per block maximum. More than 5
becomes difficult to track.
All small biopsies are wrapped in lens paper with forceps and also
unwrapped with forceps- this serves to clean the forceps when wrapping
and also when unwrapping.
Counts of # of pieces may be less than reliable since not infrequently
tissues will fragment with slicing; pictures do lie, but less often .
The combination of the two generally allows the techs to get it correct.
Extra pieces occasionally turn up or pieces are found at embedding.
Those are placed in a block and cut to see if we can ID the case.
PS We re-use disposable tissue molds until they are too grungy and then
are tossed.
Steve
Steve A. McClain, MD
McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000
More information about the Histonet
mailing list