[Histonet] Bouin's without picric acid

[Cheryl Crowder]

Tyrone - 
I have been working with fish (whole and pieces and parts) for years.  The 
fixative of choice  for whole fish as been Davidson's.  It contains 
formalin, alcohol, acetic acid and water.  After the whole fish is opened,  
the acetic acid begins to soften the harder tissues  while the formalin 
fixes the  rest.  Since there is alcohol, the tissues are then placed in 70% 
alcohol and processed.   The length of time for fixation depends on the size 
of the fish of course.  You'll just have to find the times.  Many of the 
small fish (fingerlings) have been  ready in 24 hours.  But many of the fish 
we get have been fixed for a week or more and results are really good.  
Contact me personally if you need more information.
Cheryl


Cheryl Crowder, BA, HTL(ASCP)
Crowder Histology Consulting
4952 Alvin Dark Ave.
Baton Rouge, LA  70820
(225)  772-2865