[Histonet] RE: MYO-D1

[Settembre, Dana]

Hi Donna,
I use Dako's Myo-D1 cat. M3512. Use it at 1:25
I use Dako's labeled polymer kit, Envision + Mouse.

Use Trilogy (from Cell Marque) in a steamer. That means:
Start the steamer and put a two Coplin jars in filled with Trilogy.
Place all your slides in 1st Coplin jar for 30 min. right then, at RT.
The temp will go up to 95 degrees as the Trilogy acts as a deparaffinizer.
Then put he slides in the 2nd Coplin jar for 30 min. and Trilogy acts as AR.

Good luck.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ  07103


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Donna Liaros
Sent: Friday, September 09, 2011 4:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] MYOD1

Any protocol  recommendations for MYOD1?   I am having a difficult time in trying to work up this antibody.
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