[Histonet] decal

Webb, Dorothy L Dorothy.L.Webb <@t> HealthPartners.Com
Fri Sep 9 13:14:49 CDT 2011


We use Rapid Cal Immuno (formic acid based) from BBC Biocemical out of Washington.  Works great and does not compromise IHC as name suggests!

Dorothy Webb

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, September 09, 2011 12:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 94, Issue 9

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Today's Topics:

   1. Re: P63 controls (Troutman, Kenneth A)
   2. Full Time HT position in Oklahoma City (Gaiser, Marcia)
   3. STP420 vs. Peloris (Davide Costanzo)
   4. Seeking new position (Charles O)
   5. Comment on HT/HTL (anuradha shrivastava)
   6. Facebook (Nicole Tatum)
   7. thinprep (tahseen <@t> brain.net.pk)
   8. I'm so sad for AFIP to close (Weems, Joyce)
   9. RE: I'm so sad for AFIP to close (sdysart <@t> mirnarx.com)
  10. RE: I'm so sad for AFIP to close (Edwards, Richard E.)
  11. Decal for IHC (Laudon, Heather M)
  12. RE: I'm so sad for AFIP to close (Shirley A. Powell)
  13. Floater Problem (Laurie Colbert)
  14. Re: Decal for IHC (Rene J Buesa)
  15. RE: Floater Problem (Bartlett, Jeanine (CDC/OID/NCEZID))
  16. RE: I'm so sad for AFIP to close (Kelly Cross)
  17. Re: Floater Problem (Kelly Cross)
  18. RE: Floater Problem (Bea DeBrosse-Serra)
  19. RE: Decal for IHC (Shirley A. Powell)
  20. Traveling HistoTech San Francisco Area (Heckford, Karen - SMMC-SF)
  21. RE: Floater Problem (Shirley A. Powell)
  22. RE: Decal for IHC (Sebree Linda A)


----------------------------------------------------------------------

Message: 1
Date: Thu, 8 Sep 2011 14:16:21 -0500
From: "Troutman, Kenneth A" <Ashley.Troutman <@t> Vanderbilt.Edu>
Subject: Re: [Histonet] P63 controls
To: "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <7B310892042DA74CB3590053F424CFE61441FCEE1C <@t> ITS-HCWNEM06.ds.Vanderbilt.edu>

Content-Type: text/plain; charset="us-ascii"

We use normal prostate and we have also used skin in the past-they both work well.  I would avoid pre-cutting a lot of controls, though; it has a tendency to loose antigenicity.

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232

Message: 9
Date: Thu, 8 Sep 2011 09:08:52 -0400
From: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
Subject: [Histonet] P63 controls
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
      <OF62E3D6D8.5113CF5C-ON85257905.0048390E-85257905.00483911 <@t> LocalDomain>

Content-Type: text/plain;     charset=ISO-8859-1

Hi Histonetters
We are having trouble with our control for P63 what is everyone using?

Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center sbaldwin <@t> mhhcc.org Ph 812-996-0210, 0216,  Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only.




------------------------------

Message: 2
Date: Thu, 8 Sep 2011 15:16:48 -0500
From: "Gaiser, Marcia" <Marcia_Gaiser <@t> ssmhc.com>
Subject: [Histonet] Full Time HT position in Oklahoma City
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <728F817C02110E498D803A7C3B0C62480690A37B6E <@t> S009-APEXM06.ds.ad.ssmhc.com>

Content-Type: text/plain; charset="Windows-1252"

POSITION REQUIREMENTS:

EDUCATION:   High school diploma or GED preferred.

CERTIFICATION, LICENSURE, BONDING:  Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) ? or ? other nationally recognized certifying agency acceptable to the Laboratory Director ? or ? experience acceptable to the Laboratory Director.

EXPERIENCE:  Two years of satisfactory histology experience.

SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES.  Outstanding benefits package including generous paid time off.

Apply online at www.saintsok.com<http://www.saintsok.com<http://www.saintsok.com/><https://mobile.ssmhc.com/owa/UrlBlockedError.aspx>>, Ad# 10762, or contact Christina Hughes at (405) 272-6105 for more information.

Thank you!

Christina Hughes
HR Recruiter
St. Anthony Hospital
(405) 272-6105 - phone
(405) 272-6781 - fax


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------------------------------

Message: 3
Date: Thu, 8 Sep 2011 13:37:12 -0700
From: Davide Costanzo <pathlocums <@t> gmail.com>
Subject: [Histonet] STP420 vs. Peloris
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CA+F+RhoC=fSmo4Ufjq+vv8Bwu+hC3p3Dr40x4_2vOUBNi+nqZw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Considering both options. Can any STP users out there give me opinions?
Please give reasons, not just "go with" this one. Thanks.


------------------------------

Message: 4
Date: Thu, 8 Sep 2011 17:07:35 -0500
From: Charles O <charleso.o606 <@t> gmail.com>
Subject: [Histonet] Seeking new position
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CAJvHW2+d1dERKCEDyrJ=UTXufvNVu=t04+=cwSojNeaTum8G5w <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

   Hi,
      I am a certified histotech seeking a new position. I have an extensive experience in the general histological technics and in Immunohistochemistry.
I am willing to relocate.

    Thanks,

  Charles O.


------------------------------

Message: 5
Date: Thu, 8 Sep 2011 20:55:08 -0400
From: anuradha shrivastava <shrivastavaanuradha <@t> hotmail.com>
Subject: [Histonet] Comment on HT/HTL
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT115-DS1048065F65F786EAEEB14BAD010 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Hi All,
I feel so fortunate that I studied Histo in Cobleskill, with my bio background It was perfect. I think the science sub. background is also needed specially anatomy, so that one knows how to embed different tissues. I hope u all will agree with me. Slide submittion should be there in the exam.
Anu  Shrivastava. M.S , HTL (ASCP)
Histology Team Leader,
South County Hospital
RI

------------------------------

Message: 6
Date: Fri, 9 Sep 2011 09:21:06 -0400 (EDT)
From: "Nicole Tatum" <nicole <@t> dlcjax.com>
Subject: [Histonet] Facebook
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <4934.208.62.167.196.1315574466.squirrel <@t> webmail.realpages.com>
Content-Type: text/plain;charset=iso-8859-1

Hey histonetters,

Ive created a community page for histologist on Facebook. Please join or like  Histo-ville. It a page for people to make friends, troubleshoot, or discuss issues. I hope to create a large community of friends. So stop by and say hi. I look forward to meeting fellow techs.

Nicole Tatum, HT ASCP




------------------------------

Message: 7
Date: Fri, 9 Sep 2011 18:34:25 +0500 (PKT)
From: tahseen <@t> brain.net.pk
Subject: [Histonet] thinprep
To: histonet <@t> lists.utsouthwestern.edu
Cc: cyto <@t> skm.org.pk
Message-ID: <62815.203.135.35.66.1315575265.squirrel <@t> brain.net.pk>
Content-Type: text/plain;charset=iso-8859-1

We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain.
Can you share your experience regarding both types of machines,
1) Which type of system is best
2)which machine will provide an auto stainer 3)which model of the recommended machine is best 4)will they arrange for training of cytotechnologist and pathologist for processing and reportting of specimen 5)which company is providing best after sale service Thanks





------------------------------

Message: 8
Date: Fri, 9 Sep 2011 10:33:42 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] I'm so sad for AFIP to close
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <92AD9B20A6C38C4587A9FEBE3A30E1640827A8BBEB <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

I never really thought it would happen but it has...

http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html



Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).
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------------------------------

Message: 9
Date: Fri, 9 Sep 2011 10:02:43 -0500
From: <sdysart <@t> mirnarx.com>
Subject: RE: [Histonet] I'm so sad for AFIP to close
To: <JWeems <@t> sjha.org>,  <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <D957F2A7D21959488C492A2680F9920A2964F3 <@t> SVREXCH.asuragen.us>
Content-Type: text/plain;       charset="us-ascii"

Wow...that is sad news.

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, September 09, 2011 9:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] I'm so sad for AFIP to close

I never really thought it would happen but it has...

http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat
hology-Community.html



Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Fri, 9 Sep 2011 16:07:32 +0100
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
Subject: RE: [Histonet] I'm so sad for AFIP to close
To: "'sdysart <@t> mirnarx.com'" <sdysart <@t> mirnarx.com>, "JWeems <@t> sjha.org"
        <JWeems <@t> sjha.org>, "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <7722595275A4DD4FA225B92CDBF174A101A4EC22F6DF <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"

Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sdysart <@t> mirnarx.com
Sent: 09 September 2011 16:03
To: JWeems <@t> sjha.org; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] I'm so sad for AFIP to close

Wow...that is sad news.

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, September 09, 2011 9:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] I'm so sad for AFIP to close

I never really thought it would happen but it has...

http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat
hology-Community.html



Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).
It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.

_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 11
Date: Fri, 9 Sep 2011 15:23:16 +0000
From: "Laudon, Heather M" <HMLaudon <@t> gundluth.org>
Subject: [Histonet] Decal for IHC
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <D5C20A247E19B546BB7BDD59450B37DDD0A2FD <@t> LXEXMB01.gundluth.org>
Content-Type: text/plain; charset="iso-8859-1"

Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help!



Heather Laudon, HTL

Histology Technician

Surgical Pathology Laboratory

Gundersen Lutheran Hospital

1900 South Avenue

Lacrosse, WI 54601

Mail Stop: H04-008

(608)775-3139




------------------------------

Message: 12
Date: Fri, 9 Sep 2011 11:32:47 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] RE: I'm so sad for AFIP to close
To: "Weems, Joyce" <JWeems <@t> sjha.org>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9BF995BC0E47744E9673A41486E24EE238DE9967AE <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"

I know, just breaks your heart.  I spent 3 weeks there in 19...... well a long time ago.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, September 09, 2011 10:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] I'm so sad for AFIP to close

I never really thought it would happen but it has...

http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html



Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).
It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 13
Date: Fri, 9 Sep 2011 08:40:48 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] Floater Problem
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: Debra Cobb <Debra.Cobb <@t> dako.com>
Message-ID:
        <57BE698966D5C54EAE8612E8941D76830AD2B0BA <@t> EXCHANGE3.huntingtonhospital.com>

Content-Type: text/plain;       charset="us-ascii"

I am starting a PI project concerning floaters on slides.  I am collecting data and determining whether the floater is in the block or on the slide.  If the floater is in the block, it either came from the grossing step or the embedding step.  Of course, both the PA and the embedders swear that they clean their forceps and their work surfaces diligently.  My problem is in determining where the floater actually came from.  Any suggestions??



Thanks,

Laurie Colbert



------------------------------

Message: 14
Date: Fri, 9 Sep 2011 08:45:54 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Decal for IHC
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,    Heather MLaudon
        <HMLaudon <@t> gundluth.org>
Message-ID:
        <1315583154.12504.YahooMailClassic <@t> web65711.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

EDTA pH 7
Ren? J.

--- On Fri, 9/9/11, Laudon, Heather M <HMLaudon <@t> gundluth.org> wrote:


From: Laudon, Heather M <HMLaudon <@t> gundluth.org>
Subject: [Histonet] Decal for IHC
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, September 9, 2011, 11:23 AM


Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help!



Heather Laudon, HTL

Histology Technician

Surgical Pathology Laboratory

Gundersen Lutheran Hospital

1900 South Avenue

Lacrosse, WI 54601

Mail Stop: H04-008

(608)775-3139


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 15
Date: Fri, 9 Sep 2011 15:45:08 +0000
From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
Subject: [Histonet] RE: Floater Problem
To: Laurie Colbert <laurie.colbert <@t> huntingtonhospital.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Cc: Debra Cobb <Debra.Cobb <@t> dako.com>
Message-ID:
        <DF1CBA3D83D9A344A7D6A045188E448416E3513F <@t> EMBX-CLFT1.cdc.gov>
Content-Type: text/plain; charset="us-ascii"

I have also heard of the occasional floater coming from the processor and making its way into the paraffin block.

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Friday, September 09, 2011 11:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Debra Cobb
Subject: [Histonet] Floater Problem

I am starting a PI project concerning floaters on slides.  I am
collecting data and determining whether the floater is in the block or
on the slide.  If the floater is in the block, it either came from the
grossing step or the embedding step.  Of course, both the PA and the
embedders swear that they clean their forceps and their work surfaces
diligently.  My problem is in determining where the floater actually
came from.  Any suggestions??



Thanks,

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 16
Date: Fri, 09 Sep 2011 10:49:29 -0500
From: "Kelly Cross" <KCross <@t> cvm.tamu.edu>
Subject: [Histonet] RE: I'm so sad for AFIP to close
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,    "Shirley A. Powell"
        <POWELL_SA <@t> mercer.edu>, "Joyce Weems" <JWeems <@t> sjha.org>
Message-ID: <4E69EF39020000E3000DAE6A <@t> CVM.TAMU.EDU>
Content-Type: text/plain; charset=US-ASCII

As a young histotech I felt I had "arrived" when my supervisor assigned
me to take care of the AFIP study set years ago! It is sad...


Kelly S. Cross B.S., HT (ASCP)
Medical Laboratory Supervisor
Texas A&M University
Veterinary Pathobiology
VMA bldg. 207B
College Station, TX 77843-4467
Office: 979-862-3658
Lab: 979-845-5149
Fax: 979-862-1147
kcross <@t> cvm.tamu.edu
>>> "Shirley A. Powell" <POWELL_SA <@t> mercer.edu> 9/9/2011 10:32 AM >>>
I know, just breaks your heart.  I spent 3 weeks there in 19...... well
a long time ago.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Friday, September 09, 2011 10:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] I'm so sad for AFIP to close

I never really thought it would happen but it has...

http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html



Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


Confidentiality Notice:
This e-mail, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please delete this message, and
reply to the sender regarding the error in a separate email.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 17
Date: Fri, 09 Sep 2011 10:50:32 -0500
From: "Kelly Cross" <KCross <@t> cvm.tamu.edu>
Subject: Re: [Histonet] Floater Problem
To: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Cc: Debra Cobb <Debra.Cobb <@t> dako.com>
Message-ID: <4E69EF78020000E3000DAE71 <@t> CVM.TAMU.EDU>
Content-Type: text/plain; charset=US-ASCII

Water bath????


Kelly S. Cross B.S., HT (ASCP)
Medical Laboratory Supervisor
Texas A&M University
Veterinary Pathobiology
VMA bldg. 207B
College Station, TX 77843-4467
Office: 979-862-3658
Lab: 979-845-5149
Fax: 979-862-1147
kcross <@t> cvm.tamu.edu
>>> "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com> 9/9/2011
10:40 AM >>>
I am starting a PI project concerning floaters on slides.  I am
collecting data and determining whether the floater is in the block or
on the slide.  If the floater is in the block, it either came from the
grossing step or the embedding step.  Of course, both the PA and the
embedders swear that they clean their forceps and their work surfaces
diligently.  My problem is in determining where the floater actually
came from.  Any suggestions??



Thanks,

Laurie Colbert

_______________________________________________
Histonet mailing list
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Message: 18
Date: Fri, 9 Sep 2011 08:52:52 -0700
From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
Subject: [Histonet] RE: Floater Problem
To: "'Laurie Colbert'" <laurie.colbert <@t> huntingtonhospital.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Cc: Debra Cobb <Debra.Cobb <@t> dako.com>
Message-ID:
        <493CAA64F203E14E8823737B9EE0E25F091D4EDE1F <@t> EXCHMB01.isis.local>
Content-Type: text/plain; charset="us-ascii"

Are gloves being worn during the whole process, including slide printing?

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Friday, September 09, 2011 8:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Debra Cobb
Subject: [Histonet] Floater Problem

I am starting a PI project concerning floaters on slides.  I am
collecting data and determining whether the floater is in the block or
on the slide.  If the floater is in the block, it either came from the
grossing step or the embedding step.  Of course, both the PA and the
embedders swear that they clean their forceps and their work surfaces
diligently.  My problem is in determining where the floater actually
came from.  Any suggestions??



Thanks,

Laurie Colbert

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 19
Date: Fri, 9 Sep 2011 11:55:17 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] RE: Decal for IHC
To: "Laudon, Heather M" <HMLaudon <@t> gundluth.org>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9BF995BC0E47744E9673A41486E24EE238DE996815 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"

5% formic acid

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M
Sent: Friday, September 09, 2011 11:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal for IHC

Can anyone offer a recommendation for a good decalcifier that does not comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your help!



Heather Laudon, HTL

Histology Technician

Surgical Pathology Laboratory

Gundersen Lutheran Hospital

1900 South Avenue

Lacrosse, WI 54601

Mail Stop: H04-008

(608)775-3139


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------------------------------

Message: 20
Date: Fri, 9 Sep 2011 08:55:29 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> CHW.edu>
Subject: [Histonet] Traveling HistoTech San Francisco Area
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9ECF174E7DA83046BE6EBFDE009E28A388C439 <@t> CHW-MSG-301.chw.edu>
Content-Type: text/plain;       charset="us-ascii"

Hello,

I am going to need a ASCP certified Histotech to work for me for about
1.5-2 weeks in the next 2-4 weeks.   If you now of a temp. service or
someone interested.   Call me and leave a message and will get back to
you.



Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167

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Message: 21
Date: Fri, 9 Sep 2011 11:59:45 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] RE: Floater Problem
To: Laurie Colbert <laurie.colbert <@t> huntingtonhospital.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Cc: Debra Cobb <Debra.Cobb <@t> dako.com>
Message-ID:
        <9BF995BC0E47744E9673A41486E24EE238DE99682D <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"

There is a good article in the JOH, June 2011, Vol 34, Number 2,  Gadgets for "Floaters" prevention in the Histopathology Laboratory by Izak Dimenstein that addresses ways to help prevent them.  He discusses floater sources in the discussion.

Shirley

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Friday, September 09, 2011 11:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Debra Cobb
Subject: [Histonet] Floater Problem

I am starting a PI project concerning floaters on slides.  I am
collecting data and determining whether the floater is in the block or
on the slide.  If the floater is in the block, it either came from the
grossing step or the embedding step.  Of course, both the PA and the
embedders swear that they clean their forceps and their work surfaces
diligently.  My problem is in determining where the floater actually
came from.  Any suggestions??



Thanks,

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 22
Date: Fri, 9 Sep 2011 11:26:56 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Decal for IHC
To: "Laudon, Heather M" <HMLaudon <@t> gundluth.org>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <F0B9FD4B28C80E43996FFD4408B198AEA5B9E6 <@t> UWHC-MAIL3.uwhis.hosp.wisc.edu>

Content-Type: text/plain;       charset="us-ascii"

Hi Heather,

Whatever we use here, if you remember, doesn't compromise IHC results,
even for ER/PR.  Jamie says its Protocol decalcifier B.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laudon,
Heather M
Sent: Friday, September 09, 2011 10:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal for IHC

Can anyone offer a recommendation for a good decalcifier that does not
comprimise Immunohistochemistry results...in particular ER/PR? Thanks
for your help!



Heather Laudon, HTL

Histology Technician

Surgical Pathology Laboratory

Gundersen Lutheran Hospital

1900 South Avenue

Lacrosse, WI 54601

Mail Stop: H04-008

(608)775-3139


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

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End of Histonet Digest, Vol 94, Issue 9
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