[Histonet] (no subject)

[Anatoli Gleiberman]

You can freeze samples (either fresh or formalin fixed, but in later case with sucrose cryoprotection) in 7% gelatin in PBS instead of OCT. Sometimes it gives much better conditions for cutting as well.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost)
Sent: Tuesday, September 06, 2011 5:55 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi all

I have to prepare frozen slides of zebra fish, but it is not permitted to use OCT because of the presence of different polymers in the OCT compound. Is anyone of you familiar with the preparation of frozen slides without using OCT?

Thanks

Joost Bruijntjes
Zeist
Holland

TNO.NL<http://www.tno.nl/>

Joost Bruijntjes

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