[Histonet] New Ventana Kappa and Lambda Probes

[Dorsey, Holly L]

Was wondering if anyone would be willing to share their protocols for the Ventana Kappa and Lambda ASR probes for the Benchmark XT.  I have been having a time trying to get these protocols worked out.  When I ran the probes the first couple of times with the same protocol as the old probes, they were beautiful.  The probes then stopped working all together with no staining at all.  I adjusted the stringency wash temp and now I have a ton of background staining.  Any suggestions would be greatly appreicated.

Thanks,

Holly L. Dorsey, HT  CMC-NE, Concord NC

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