[Histonet] Problems with Frozen tissues

[Kim Donadio]

Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. 
 
Kim


________________________________
From: Igor Deyneko <igor.deyneko <@t> gmail.com>
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Monday, October 31, 2011 9:56 AM
Subject: [Histonet] Problems with Frozen tissues

I'm looking for some advice on frozen tissues. This is the first time I'm
doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut
well onto special Gold Plus slides from Fisher. Then, when I was ready to
stain the slides, i air dried them fro an hour and wanted to do H&E and
Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on
preventing this mischief?
Thank you in advance.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
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