[Histonet] saving slides between levels for IHC?

[Kim Donadio]

I think it depends on what the specimen is. All of the places Ive worked have always kept extras(levels) on sentinal nodes for keratins, also for prostate needle biopsies for p63/504s or other IHC's. 
 
Really doesnt it come down to what the Pathologist needs to make a diagnosis for the patient? 
 
I know it's a pain to cut so many levels and save them. Its also costly. But trust me, none of that is as bad as the sinking feeling you get when a biopsy has been exhausted and they need more stains :(
 
Hope this helps. Just my 2 cents worth.......
 
Kim Donadio


________________________________
From: Sheila Fonner <sfonner <@t> labpath.com>
To: "'Harrison, Sandra C.'" <Sandra.Harrison3 <@t> va.gov>; histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, October 26, 2011 7:28 AM
Subject: RE: [Histonet] saving slides between levels for IHC?

Sandy,

I don't think it is a "standard" procedure.  However, our pathologist will
frequently ask us to keep intervening levels for possible IHC on certain
cases that are small or where the tumor is about to be exhausted.  We are a
dermatopathology lab.  When it is requested, we cut the whole case on a
clean waterbath, using distilled water and no additives, on plus slides.
The routine H&E's are stained normally and the other slides are dried and
held for possible immunos.

Sheila
KDL Pathology
Knoxville, TN


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Harrison,
Sandra C.
Sent: Tuesday, October 25, 2011 2:00 PM
To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis <@t> mayo.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] saving slides between levels for IHC?

Does anyone know if it is standard  to save sections from between the
levels for IP?  Is it standard at your institution?





Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449



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