[Histonet] RE: DRYING TIMES for immuno stains

Goins, Tresa TGoins <@t> mt.gov
Thu Oct 20 10:00:27 CDT 2011


Air dry over air vent for a minimum of 30 min
Into 60C oven for 15 min
Direct transfer to hot water-detergent bath for de-wax


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Thursday, October 20, 2011 6:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DRYING TIMES for immuno stains

Can you tell me how long you let your IHC slides air dry at room
temperature  and how long you put them in the oven prior to staining?

 

Thanks

Diana

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