[Histonet] RE: Von Kossa Stain for calcium

[Mayer,Toysha N]

When running more than 5-10 slides at a time in a silver stain, a precipitate will most likely form.  It would be better to run them in small batches in coplin jars to give you the necessary control needed.  
Acid washing is fine, but just laboratory grade cleaner works as well.
I usually place the slides in a coplin jar, under a lamp wrapped in foil, on a warm surface (hot plate, embedder, or even in a waterbath).For 35 slides I would stagger the start times to allow myself time to check them. The only extra step would be having multiple control slides. 
Running 24 slides at once can be daunting, so a few in multiple coplin jars would work better for me to handle.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org





Message: 17
Date: Tue, 4 Oct 2011 10:36:30 -0400
From: "Sherwood, Margaret" <MSHERWOOD <@t> PARTNERS.ORG>
Subject: Re: [Histonet] Von Kossa Stain for calcium
To: <histonet <@t> lists.utsouthwestern.edu>
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We are a research lab and don't do a big volume of special stains.  We just
received a project for 35 slides that need Von Kossa Stain.
I acid-washed some small glass staining dishes-no rack required (in the past, we
only had a few requests).  

Can I run slides in plastic slide holders (24-place) in an acid-washed beaker
for the silver nitrate step?

Any tips would be appreciated.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org



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