[Histonet] decal question

[Edwards, Richard E.]

Was it decalcifying a tissue fixed in a formalin based fixative using a  hydrochloric acid containing decalcifying fluid? as even storage of HCl and formalin in the same area is a bad plan.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald
Sent: 04 October 2011 12:47
To: Patsy Ruegg
Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decal question

Was there more to the question?  You would not want to use any fixatives 
containing metals, such as mercury, if you will be doing end-point 
decalcification determination using x-rays.




"Patsy Ruegg" <pruegg <@t> ihctech.net> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/03/2011 11:27 AM

To
"'Sarah Dysart'" <sdysart <@t> mirnarx.com>, 
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] decal question






I think I miss phrased this question, we thought they were asking what
fixative should never be used if you are planning to do acid decal after
fixation?  We still think it must be osmium???? 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: Sarah Dysart [mailto:sdysart <@t> mirnarx.com] 
Sent: Monday, October 03, 2011 11:55 AM
To: Patsy Ruegg; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] decal question

Formalin?  Isn't all decal acid decal?

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy 
Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question

Hi Everyone,

 

I have a new student taking course work at UND and using my lab for her
practical clinical site.  She took a test on decal today and there was a
question we didn't know the answer to.

 

What fixative should not be used for acid decalcification?  Would it be
osmium tetroxide?

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
web site  <http://www.ihctech.net> www.ihctech.net

 


This email is confidential and intended solely for the use of the 
Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that 
is
privileged & confidential within the meaning of applicable law. 
Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. 
If
you are NOT the intended recipient please contact the sender and dispose 
of
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