[Histonet] demonstration of asbestos by means of electron microscopy

Emin Öztaş eminoztas <@t> gata.edu.tr
Thu Nov 24 08:49:30 CST 2011

We showed aspestos fibers with EM by using a formvar coated 200 mesh grids. 1 micro liter sample solution was put on the surface of the formwar coated grid. The grid was dried at room temperature and directly searched with EM. 

----- Orijinal Mesaj -----
Kimden: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
Kime: histonet <@t> lists.utsouthwestern.edu, "Yolanda Davies" <yolanda.davies <@t> uct.ac.za>
Gönderilenler: 22 Kasım Salı 2011 16:28:29
Konu: Re: [Histonet] demonstration of asbestos by means of electron	microscopy

Many years ago asbestos fibers were demonstrated in hypochlorite digested tissues. The remaining material was filtered through Milllipore filter, washed and the fibers observed with polarized light.
The Perl's method will stain no the asbestos fibers (that cannot be stained) but a reactive capsule the tissues secrete around the fiber, so it is an indirect method based in: if there is a tissue reaction to asbestos, they should have been present at a certain moment.
As to its demonstration with EM I do not know but I think it will be very difficult to infiltrate the fibers in Epoxi resin to be cut. If you are able to do that: how are you going to identify an extremely thin section of asbestos?
I think that you should either try the tissue digestion or give another try at the Perl reaction.
René J.

--- On Tue, 11/22/11, Yolanda Davies <yolanda.davies <@t> uct.ac.za> wrote:

From: Yolanda Davies <yolanda.davies <@t> uct.ac.za>
Subject: [Histonet] demonstration of asbestos by means of electron microscopy
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, November 22, 2011, 4:14 AM

Dear all

I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not

Is it possible to reveal asbestos by means of electron microscopy?

Usually asbestos is demonstrated using the Perl's Prussian blue
technique, but most times they are elusive.
Could it be because of the sampling site or simply the nature of the

Thank you in advance

Yolanda Davies
Department of Forensic Medicine and Toxicology
Falmouth building
Anzio Road
Cape Town 
South Africa



This e-mail is subject to the UCT ICT policies and e-mail disclaimer
published on our website at
http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from
+27 21 650 9111. This e-mail is intended only for the person(s) to whom
it is addressed. If the e-mail has reached you in error, please notify
the author. If you are not the intended recipient of the e-mail you may
not use, disclose, copy, redirect or print the content. If this e-mail
is not related to the business of UCT it is sent by the sender in the
sender's individual capacity.


-----Inline Attachment Follows-----

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

Emin Oztas, MD. PhD. 
Gulhane Military Medical Academy 
Department of Medical Histology and Embryology 
Ankara, 06018, Turkey 
eminoztas <@t> gata.edu.tr 
+90 312 3043536 
+90 532 5215952 

More information about the Histonet mailing list