[Histonet] thick paraffin sections

[mesruh turkekul]

Dear Friends,


I am trying to section 25-50um thick paraffin sections of mouse brain. I
had success with 40um thick tumor sections but brain is very difficult to
section as such thick sections. I always use ParaPlast plus melting at 56 C.
Any suggestions on how to cut thick paraffin sections? Is low melting
paraffin going to help?

Thanks,


Happy holidays!

Mesruh Turkekul
mskcc.org