[Histonet] Re: To cut or not to cut mouse brains

[Donna J Emge]

I have had folding and bubbles occur periodically with PFA fixed, sucrose cryoprotected frozen brain tissue I receive from other labs. I streak a distilled water moistened brush across the slide then pick up the frozen section on the moistened area. It comes out flat and smooth every time. I let the section dry as usual, then store in -20°C or -80°C.  For ISH I use a RNase zapped and DEPC rinsed brush and streak DEPC water across the slide with the moistened brush. I section the PFA fixed, sucrose cryoprotected tissue around -21°C. Researchers report good subsequent IHC and ISH results and bring more samples.


Donna J. Emge, ASCP-HT
Mouse Histology and Phenotyping Laboratory Manager
Northwestern University
Olson Pavilion 8-333
710 North Fairbanks Court
Chicago, IL  60611
d-emge <@t> northwestern.edu<mailto:d-emge <@t> northwestern.edu>
312-503-2679