[Histonet] RE: Histonet Digest, Vol 96, Issue 27
Paul Firnschild
Paul <@t> Firnschild.com
Fri Nov 18 10:10:09 CST 2011
Solomon:
Regarding your question of how to know if the microtome advance amount.
Verifying this measurement is part of the service I provide as a service
technician. I typically do this once of twice a year as part of the routine
microtome maintenance service. Please contact me if I can be of service to
you.
Paul M. Firnschild
QA Support Services, Inc.
(404) 291.3715
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Sent: Thursday, November 17, 2011 10:39 PM
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Subject: Histonet Digest, Vol 96, Issue 27
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Today's Topics:
1. RE: Histonet Digest, Vol 96, Issue 26 (Freeman, Carol)
2. to cut or not to cut mouse brains (McLaughlin, Terry )
3. RE: to cut or not to cut mouse brains (Helen Fedor)
4. Re: to cut or not to cut mouse brains
(Grantham, Andrea L - (algranth))
5. RE: to cut or not to cut mouse brains (Connolly, Brett M)
6. RE: to cut or not to cut mouse brains (Connolly, Brett M)
7. RE: to cut or not to cut mouse brains (Helen Fedor)
8. HTL Lead Job Opening (Morocho, Jennifer)
9. Brass cryostat chucks (Goodwin, Diana)
10. Source of IgG4 for Isotype control (Ladd, Sharron)
11. Re: Brass cryostat chucks (Grantham, Andrea L - (algranth))
12. AUTO: is out of the office. (returning Wed 10/19/2011)
(Christina.Wilson <@t> leica-microsystems.com)
13. microtomes (Salomao Segal)
14. broken chuck holder (Cheryl)
15. RE: Paraplast X-tra (connie grubaugh)
16. Re: Paraplast X-tra ( cls71877 <@t> sbcglobal.net )
17. RE: RE: H. Pylori - IHC (Karen Lahti)
----------------------------------------------------------------------
Message: 1
Date: Thu, 17 Nov 2011 13:14:58 -0500
From: "Freeman, Carol" <Carol.Freeman <@t> utoledo.edu>
Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 26
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D8839F381A97B045BA5A607F5EEDF311018B52F4 <@t> MSG02CV00.utad.utoledo.edu>
Content-Type: text/plain; charset="us-ascii"
In response to the broken chuck holders, I have seen it twice and both
times it is with a tech who uses chemicals on blocks (RDO, FORMICAL or
AMMONIA WATER ETC....) and then not rinse the blocks before putting them
on the chuck holder. The chemicals erode the metal mechanism holding
the spring and it will eventually snap. Have the tech rinse the blocks
in water after taking them out of whatever chemical used before putting
them on chuck holder and it should hopefully help. I hope that helps....
Carol E. Freeman HTL (ASCP) B.S.
Department of Pathology
University of Toledo Medical Center
3000 Arlington Avenue
Toledo, OH 43614-5807
carol.freeman <@t> utoledo.edu
(419)383-5639
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Sent: Thursday, November 17, 2011 1:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 96, Issue 26
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When replying, please edit your Subject line so it is more specific
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Today's Topics:
1. Paraffin slides storage (Itai Moshe)
2. RE: Fatty mamma tissue keeps on washing off (Hoekert, W.E.J.)
3. RE: Histonet Digest, Vol 96, Issue 24 reprocessing
(Steve McClain)
4. RE: Histonet Digest, Vol 96, Issue 24 reprocessing Other
methods (Steve McClain)
5. RE: Histonet Digest, Vol 96, Issue 24 reprocessing
(Steve McClain)
6. broken chuck holder (yesyes <@t> comcast.net)
7. RE: broken chuck holder (Essex, David)
8. Re: broken chuck holder (Paula Sicurello)
9. RE: H. Pylori - IHC (Blazek, Linda)
10. RE: H. Pylori - IHC (Tom McNemar)
11. Manual or parts list for a Lipshaw autopsy table (Glen Dawson)
12. IHC (Sarah Dysart)
13. RE: IHC (Elizabeth Chlipala)
14. RE: RE: Validation (Amber McKenzie)
----------------------------------------------------------------------
Message: 1
Date: Thu, 17 Nov 2011 09:47:22 +0200
From: Itai Moshe <itai.moshe <@t> mail.huji.ac.il>
Subject: [Histonet] Paraffin slides storage
To: histonet <@t> lists.utsouthwestern.edu
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<CACp9T1rhAeauYsY=mb0cXsPO-F=1+8k842xhpcVx48B5r_caPA <@t> mail.gmail.com>
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Hello Histonets,
I have some questions about paraffin slides storage.
1) For how long do you store paraffin slides after sectioning ?
2) At what conditions do you store the slides ?
Itai
------------------------------
Message: 2
Date: Thu, 17 Nov 2011 09:09:19 +0100
From: "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl>
Subject: RE: [Histonet] Fatty mamma tissue keeps on washing off
To: "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1190CB05C44B13409483514729C2FC3601F841EC <@t> PAIT42.olvg.nl>
Content-Type: text/plain; charset="iso-8859-1"
Hi histonetters
Here is an update on my struggle to keep the fatty mamma tissue on the
slides. I got a few responses on my mail so I have tried a few other
things.
Post fix the slides. Deparaffinize the slides using xylene and hydrate
to water, post fix the slides in 10% NBF for 10 minutes (I did it for
about 4 hours) and proceed as usual. That worked for a few of my
hopeless cases. Still, some other cases would still come of the slides.
I have also tried the Dako slides. Indeed they work great. Of the 4
cases that washed off no matter what, all but one stayed on the Dako
slides.
So from now on, if we have a case that washes off, we will be using the
Dako slides.
Thanks for your help,
Willem Hoekert
OLVG AMsterdam
The Netherlands
________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Hoekert, W.E.J.
Verzonden: do 20-10-2011 12:09
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] Fatty mamma tissue keeps on washing off
Hi Histonetters,
Every once in a while, we have mamma tissue that washes off the slides
completely. The reason for this is that the tissue is still too fat
and/or not sufficiently fixed. There is no problem with the HE stain,
but the immuno will wash off. What do you do in such cases?
I have tried the following:
1: Baking the slides for about 3/4 hours on superfrost slides (that
works sometimes but not always)
2: Wood glue slides (that works sometimes but not always)
3:We put the blocks back in hot parraffin for several hours (that
usually works) but I was told that this will have a bad influence on the
Her2neu stain: a 2+ could become a 1+.
I know, the solution is to be sure that the tissue is fixed long enough
and that the fat has been removed sufficiently using acetone, but
sometimes that is just not the case. How do you cope with this?
Willem Hoekert
PA-lab
OLVG Amsterdam
The Netherlands
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Disclaimer:
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Verstrekking aan en gebruik door anderen dan geadresseerden is niet
toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de
verzender hiervan op de hoogte te stellen en het bericht te verwijderen.
In verband met electronische verzending kunnen aan dit e-mail bericht
geen rechten worden ontleend.
------------------------------
Message: 3
Date: Thu, 17 Nov 2011 11:17:38 +0000
From: Steve McClain <SteveM <@t> mcclainlab.com>
Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<012ADA4B5CC00F4AB5E4BAA399E0A5DF137756 <@t> ML1.McClainLabs.local>
Content-Type: text/plain; charset="us-ascii"
This sounds like a complicated problem- Generally fixed tissues left in
the processor overnight can just be re-hydrated in formalin for a few
hours and then run (we've made that mistake)
Exceptions abound in histology methods, but Unless the tissue was
well-fixed to begin with, Re-processing generally stinks.
Given that this is research and a one of a kind, it may still be worth
trying.
I would not expect Immunostains and other high-end work to be reliable
even if you do get decent sections.
First INVESTIGATE and determine what likely happened- Study the slides
and see what is wrong -are they sunken and shriveled as if paraffin did
not infiltrate?
Second Study the cut surface of the blocks with a 10x lens or dissecting
scope and determine if there was a processing failure also, any reason
to suspect reagents needed changing?
Your tissues sound maybe under-fixed (shrinkage described), probably
dehydrated and then fixed by coagulation in the alcohols on the
processor.
Is it possible that the cleaning cycle was run before you started
processing the next day?
Third try Luna's method given by Shelly and do it manually and at room
temp
Fourth buy lunch for the investigator and meet to apologize and work out
the details of fixation and any other issues discovered from your 'NTSB
Accident Investigation'.
Who knows, it may work.
Fifth, assign one person to supervise (be in charge of) processing and
have them keep statistics ( I have a large graph outside my door with 1
months data on each processor, graphing # of blocks for each run, when
reagents were replenished/changed, noting every failure)- even in a
small lab like ours, once we got to 6 processors running 1-4 times a
day, someone needs to be in charge and held responsible.
Six, hang a sign on the exit door (like the one on my back door) "DID
YOU START THE TISSUE PROCESSOR?"
Some may think that is over-reacting to a one-time error; when it
happens a second time you earned and deserve the reputation.
Be thankful it did not harm any patients- in the accident world that is
known as a near-miss.
Good luck
Steve A. McClain, MD
631 361 4000
Message: 9
Date: Wed, 16 Nov 2011 15:05:38 +0000
From: Shelly Christenson <christen <@t> vet.k-state.edu>
Subject: RE: [Histonet] is there a way to recover ruined tissues
To: Patsy Ruegg <pruegg <@t> ihctech.net>, 'histo net'
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<06E342B6098ED9478347E1407764C80404B13411 <@t> VETMXHT.ads.vet.k-state.edu>
Content-Type: text/plain; charset="us-ascii"
Patsy,
I have used this reprocessing schedule from Lee Luna's book
"Histopathologic Methods and Color Atlas of Specials Stains and Tissue
Artifact". This procedure has worked for me on a couple of occasions,
You may need to change the times a little since your tissue samples are
so small. Luna's book has a lot of information that is very helpful, and
would be a good reference book to have in the lab. Hope this helps you
out, give it a try.
Tissue Softening Solution
The following solution is used for softening dried tissue specimens
prior to reprocessing or processing
Formol-Sodium Acetate (Stock) Solution
Concentrated formalin (37%) solution 10 ml
Sodium acetate 2 gm
Tap water 90 ml
Formol-Glycerol (Working) Solution
Formalin-sodium acetate (stock) 90 ml
Glycerol (glycerin) 10 ml
Reprocessing Schedule
Melt the paraffin blocks down
Place the tissue in 3 changes of xylene for 1 hour each
Place in 100% alcohol, 2 changes for 1 hour each
Place in 95% alcohol, 2 changes for 1 hour each
Place under running tap water for 20 minutes
Place in formol-glycerol working solution until tissue becomes soft (In
most instances this will occur within 5-8 hours: extended exposure to
the formol-gycerol will not harm tissue specimens)
Place the tissue in the process and proceed with routine Processing
schedule. How ever it is not necessary for the tissue to go through the
fixative stations on the processor
Best of Luck,
Shelly
------------------------------
Message: 4
Date: Thu, 17 Nov 2011 11:23:59 +0000
From: Steve McClain <SteveM <@t> mcclainlab.com>
Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24
reprocessing
Other methods
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<012ADA4B5CC00F4AB5E4BAA399E0A5DF13779E <@t> ML1.McClainLabs.local>
Content-Type: text/plain; charset="us-ascii"
Not appropriate for your current mamafufu,
But here are two 're-processing' methods used in our lab for small fixed
tissues.
Method 1 where the tissue was bit too large for a short processing cycle
and the center of the block is sub-optimally infiltrated or soft and
needs more paraffin time.
Cutting can be improved by melting the block in a tissue mold, then
change the paraffin and allow to sit for 30-60 minutes and re-embed in
new paraffin.
Method 2 Cleaning cycle method
where small FIXED tissues were processed on too short a cycle, e.g., 4
mm punch biopsy on a 1 hour process (instead of a 4 -6 hour process)
we have used this method for taking paraffin blocks back to alcohol.
Melt blocks in correctly-sized molds in embedding center.
Re-wrap tissues (carefully) and place back into cassettes and verify
each lid is closed.
Place in VIP-type processor with an automated cleaning cycle.
Replace the purges with clean reagents.
Run the cleaning cycle through the purge xylene-alcohol on the tissue
processor, when completed- do not run through the water purge.
Re-process (correctly) beginning in 95% alcohol.
Steve A. McClain, MD
631 361 4000
------------------------------
Message: 5
Date: Thu, 17 Nov 2011 11:39:32 +0000
From: Steve McClain <SteveM <@t> mcclainlab.com>
Subject: [Histonet] RE: Histonet Digest, Vol 96, Issue 24 reprocessing
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<012ADA4B5CC00F4AB5E4BAA399E0A5DF1377AD <@t> ML1.McClainLabs.local>
Content-Type: text/plain; charset="us-ascii"
I just talked to Joel who reminded me.
Mouse livers are often hard/ brittle, even under the best of
circumstances.
Avoid heat when processing, Room temp only
Gradual dehydration 50/50/70/95% alc. 1 Hour per station.
Steve
631 361 4000
------------------------------
Message: 6
Date: Thu, 17 Nov 2011 11:40:34 +0000 (UTC)
From: yesyes <@t> comcast.net
Subject: [Histonet] broken chuck holder
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1443769015.1841423.1321530034020.JavaMail.root <@t> sz0175a.westchester.pa.m
ail.comcast.net>
Content-Type: text/plain; charset=utf-8
Does anyone have any experience with broken chuck holders? I have a tech
that has snapped 3 chuck holders in little over two years. I have never
seen this, has anyone else??
------------------------------
Message: 7
Date: Thu, 17 Nov 2011 11:42:37 -0000
From: "Essex, David" <David.Essex <@t> kingstonhospital.nhs.uk>
Subject: RE: [Histonet] broken chuck holder
To: <yesyes <@t> comcast.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20111117114238.813D1448F2B <@t> nhs-pd1e-esg108.ad1.nhs.net>
Content-Type: text/plain; charset="us-ascii"
Only when I used to work with the Incredible Hulk.....
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
yesyes <@t> comcast.net
Sent: 17 November 2011 11:41
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] broken chuck holder
Does anyone have any experience with broken chuck holders? I have a tech
that has snapped 3 chuck holders in little over two years. I have never
seen this, has anyone else??
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------------------------------
Message: 8
Date: Thu, 17 Nov 2011 07:35:53 -0500
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: Re: [Histonet] broken chuck holder
To: "Essex, David" <David.Essex <@t> kingstonhospital.nhs.uk>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAPSjddZ8wASyY8Vrhkt9xwtb+hGX+5j8WD=yEHrFP84VZbXXzw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
You should observe this tech to see what he/she is doing to break 3 in 2
years.
Then you can have a training session in the proper use of the chuck.
Either that or let them know that eating spinach before cutting is not
a good idea....
On Thu, Nov 17, 2011 at 6:42 AM, Essex, David
<David.Essex <@t> kingstonhospital.nhs.uk> wrote:
> Only when I used to work with the Incredible Hulk.....
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> yesyes <@t> comcast.net
> Sent: 17 November 2011 11:41
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] broken chuck holder
>
> Does anyone have any experience with broken chuck holders? I have a
tech
> that has snapped 3 chuck holders in little over two years. I have
never
> seen this, has anyone else??
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> This email is covered by the Kingston Hospital NHS Trust email
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------------------------------
Message: 9
Date: Thu, 17 Nov 2011 07:37:37 -0500
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: [Histonet] RE: H. Pylori - IHC
To: "'Beth.Fye <@t> HCAhealthcare.com'" <Beth.Fye <@t> HCAhealthcare.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5A2BD13465E061429D6455C8D6B40E39137A4A0770 <@t> IBMB7Exchange.digestivespeci
alists.com>
Content-Type: text/plain; charset="us-ascii"
We use BioCare's H.Pylori for IHC and it is consistency is excellent.
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Beth.Fye <@t> HCAhealthcare.com
Sent: Wednesday, November 16, 2011 5:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H. Pylori - IHC
Does anyone have recommendations for a good vendor for H.Pylori for
IHC? We have tried many, but our pathologists complain that they are
not specific for H.Pylori and that all gram negative bacteria are
staining. Currently we are not running this but our pathologists are
interested in it.
Thanks!
Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office: (804)228-6564
fax: (804)323-8638
<mailto:Beth.Fye <@t> hcahealthcare.com>
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------------------------------
Message: 10
Date: Thu, 17 Nov 2011 08:30:24 -0500
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] RE: H. Pylori - IHC
To: "'Beth.Fye <@t> HCAhealthcare.com'" <Beth.Fye <@t> HCAhealthcare.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E9A90E28259D2F4E84308C5E8EA8F7B45AB736F2FC <@t> lmhs-exchange.lmhealth.org>
Content-Type: text/plain; charset="us-ascii"
We get beautiful staining with Dako's concentrate on the Benchmark XT.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Beth.Fye <@t> HCAhealthcare.com
Sent: Wednesday, November 16, 2011 5:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H. Pylori - IHC
Does anyone have recommendations for a good vendor for H.Pylori for
IHC? We have tried many, but our pathologists complain that they are
not specific for H.Pylori and that all gram negative bacteria are
staining. Currently we are not running this but our pathologists are
interested in it.
Thanks!
Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office: (804)228-6564
fax: (804)323-8638
<mailto:Beth.Fye <@t> hcahealthcare.com>
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------------------------------
Message: 11
Date: Thu, 17 Nov 2011 09:35:34 -0600
From: Glen Dawson <ihcman2010 <@t> hotmail.com>
Subject: [Histonet] Manual or parts list for a Lipshaw autopsy table
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT106-W189FFC2289DC49A766C7B2CCC70 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
All,
I was hoping that someone could help me locate an owner's manual or
parts list for a Lipshaw Model LM-5-A Autopsy Table. It seems like the
hydraulics are going out, but we need the manual to try to address the
problem.
Thanks In Advance,
Glen Dawson BS, HT(ASCP) & QIHC
Janesville, WI
------------------------------
Message: 12
Date: Thu, 17 Nov 2011 16:20:53 +0000
From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: [Histonet] IHC
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8A70A9B2ECDD084DACFE6C59FCF86D5001FC62 <@t> SN2PRD0702MB110.namprd07.prod.ou
tlook.com>
Content-Type: text/plain; charset="us-ascii"
So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a
positive control for both of these at the same time? Will tonsil pop
positive for both?? If not, what are good controls for each of them?
Thanks
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
------------------------------
Message: 13
Date: Thu, 17 Nov 2011 09:23:35 -0700
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: IHC
To: 'Sarah Dysart' <sdysart <@t> mirnarx.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE011380AC70D1 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Tonsil is the control we use
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarah
Dysart
Sent: Thursday, November 17, 2011 9:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC
So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a
positive control for both of these at the same time? Will tonsil pop
positive for both?? If not, what are good controls for each of them?
Thanks
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 17 Nov 2011 16:41:49 +0000
From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: RE: [Histonet] RE: Validation
To: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>, 'Kim Donadio'
<one_angel_secret <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC063249 <@t> JERRY.Gia.com>
Content-Type: text/plain; charset="us-ascii"
So can you do validation backwards? Stain the slides on the new
instrument 1st and then stain on the old one to compare instead of
looking up old cases to re-run on the new instrument? I have an XT
(old) and Ultra (new).
From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsfmedctr.org]
Sent: Wednesday, November 16, 2011 4:42 PM
To: 'Kim Donadio'; Amber McKenzie; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Validation
Kim wrote: "Seems the actual amount of validation slides can vary with
different Medical directors( Pathologist)"
Yes, validation is simply the process of proving to yourself and anyone
else (pathologist, clinician, inspector, patient, lawyer) that the
product works as intended. Medical Directors are responsible for
determining what that entails, whether large or small sets of tissue.
There are certain recommendations that have been written up but it all
comes down to being able to convince anyone who looks into it that
everything is well done.
CLIA regulations outline the process and following it will ensure you
are on the right track.
I have some documents up on a Yahoo groups page that anyone can
download. They are from a validation presentation at NSH and ASCP
meetings, including the presentation powerpoint outling CLIA and CAP
requirements, model validation procedure and model validation
documentation forms. You have to join the group but it is free and I
promis there will be no emailings to bug you.
Go to Yahool groups and search for "Histoinfo" group
Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
________________________________
From: Kim Donadio [mailto:one_angel_secret <@t> yahoo.com]
Sent: Wednesday, November 16, 2011 12:42 PM
To: Morken, Timothy; 'Amber McKenzie'; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Validation
Seems the actual amount of validation slides can vary with different
Medical directors( Pathologist) or thats what Ive seen in places. They(
The Pathologist) sign off on it. With that said ER/PR and Her2 have more
vigorous criteria( very specific, such as the 25-50). Also, the 2011
AP133 guidline is suppose to go into more detail on this. I havnt seen
that new recomendation yet. So If anyone has it, would love to see it
posted here myself?
Thanks
Kim
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
To: 'Amber McKenzie' <amber.mckenzie <@t> gastrodocs.net>;
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, November 16, 2011 2:08 PM
Subject: [Histonet] RE: Validation
Amber, is this new instrument the same kind as your old instrument and
are you using the same reagents? Or is it a totally different platform
with different reagents?
Putting in a new instrument of the same kind and using the same reagents
just requires verifying the new instrument works. You have to convince
whoever wants to know that it works the same as the older instrument so
running a variety of antibodies and procedures on it will suffice to
prove that. Doing reproducibility tests also proves the instrument works
as intended - 5 to 10 identical slides on one run, 5 to 10 identical
slides on 5 to 10 runs.
If it is a totally different platform with different reagents then you
are in for revalidating each of your antibodies on the new system.
Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
-----Original Message-----
From:
histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.
utsouthwestern.edu>
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounce
s <@t> lists.utsouthwestern.edu>] On Behalf Of Amber McKenzie
Sent: Wednesday, November 16, 2011 10:21 AM
To:
histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.e
du>
Subject: [Histonet] Validation
Just wondering what y'alls opinion is on validation: I don't really
understand why optimization isn't enough. At that point, the
pathologist has said what he wanted the stain to look like, so why do
3-10 positive slides on the new instrument to compare to previous slides
from another instrument? Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.e
du>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.e
du>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 96, Issue 26
****************************************
------------------------------
Message: 2
Date: Thu, 17 Nov 2011 14:24:46 -0500
From: "McLaughlin, Terry " <tmclaugh <@t> NEMOURS.ORG>
Subject: [Histonet] to cut or not to cut mouse brains
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0B0238D44CE7C04E9D32455C8643D893E3FC0A <@t> wlmmsx02.nemours.org>
Content-Type: text/plain; charset="us-ascii"
Hello All,
I am having trouble cutting frozen mouse brains and was wondering if
someone can offer some help. The mouse was perfused in 4% PFA , the
brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
for 20 hrs, until the brain sank in this solution. It was frozen back by
using a culture plate floating in liquid nitrogen with OCT .
The problem I am having is many folds, and air bubbles under the tissue.
What I have done so far:
Cut at temp of -25, then tried - 22 and -17.
Cut at 10um, then tried 16-25um.
Tried making the knife colder by adding dry ice to it for a few seconds.
Added dry ice to sample for a couple seconds.
The sample cuts fine, it appears to happen when picking up on a slide. I
tried picking up by starting at one side or end and letting tissue
spread onto slide. Did not work.
Also tried gently laying slide on top of sample and removing
gently-still folds and air bubbles. Any help would be greatly
appreciated as we have 6 more to do.
Signed,
Help!
Terry Kokas
------------------------------
Message: 3
Date: Thu, 17 Nov 2011 19:51:57 +0000
From: Helen Fedor <hfedor <@t> jhmi.edu>
Subject: [Histonet] RE: to cut or not to cut mouse brains
To: "'McLaughlin, Terry '" <tmclaugh <@t> NEMOURS.ORG>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BCE07FB3A86FD040969A9EC0DD3EDA0D0B881C51 <@t> JHEMTMWEX4.win.ad.jhu.edu>
Content-Type: text/plain; charset="us-ascii"
Hello Terry,
These are very difficult. The colder the better. (-27). keep the slides in
the cryostat. Cut the section. Place section on a cold slide, and keep the
slide in the croystat during the following procedure.
Hold slide in left hand and brush in right. Place finger under one edge of
the section and it will start to thaw, anchoring it to the slide. Gently
brush out folds as you continue to advance the thaw line with your fingers
under the slide. It takes a bit of practice, and not really perfect. But it
works.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McLaughlin,
Terry
Sent: Thursday, November 17, 2011 2:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] to cut or not to cut mouse brains
Hello All,
I am having trouble cutting frozen mouse brains and was wondering if
someone can offer some help. The mouse was perfused in 4% PFA , the
brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
for 20 hrs, until the brain sank in this solution. It was frozen back by
using a culture plate floating in liquid nitrogen with OCT .
The problem I am having is many folds, and air bubbles under the tissue.
What I have done so far:
Cut at temp of -25, then tried - 22 and -17.
Cut at 10um, then tried 16-25um.
Tried making the knife colder by adding dry ice to it for a few seconds.
Added dry ice to sample for a couple seconds.
The sample cuts fine, it appears to happen when picking up on a slide. I
tried picking up by starting at one side or end and letting tissue
spread onto slide. Did not work.
Also tried gently laying slide on top of sample and removing
gently-still folds and air bubbles. Any help would be greatly
appreciated as we have 6 more to do.
Signed,
Help!
Terry Kokas
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 17 Nov 2011 11:57:29 -0800
From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
Subject: Re: [Histonet] to cut or not to cut mouse brains
To: "McLaughlin, Terry" <tmclaugh <@t> NEMOURS.ORG>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DB12F237-5CDD-4C16-8CD7-C6E4374967A5 <@t> email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"
I'm interested in this answer because this has happened to me - but not
always.
I usually cut brains at warmer temps. like -17 or -18 as opposed to -25 and
they are usually handled in the same manner before being brought to the lab
frozen in OCT.
Andi
On Nov 17, 2011, at 12:24 PM, McLaughlin, Terry wrote:
> Hello All,
> I am having trouble cutting frozen mouse brains and was wondering if
> someone can offer some help. The mouse was perfused in 4% PFA , the
> brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
> for 20 hrs, until the brain sank in this solution. It was frozen back by
> using a culture plate floating in liquid nitrogen with OCT .
> The problem I am having is many folds, and air bubbles under the tissue.
> What I have done so far:
> Cut at temp of -25, then tried - 22 and -17.
> Cut at 10um, then tried 16-25um.
> Tried making the knife colder by adding dry ice to it for a few seconds.
> Added dry ice to sample for a couple seconds.
> The sample cuts fine, it appears to happen when picking up on a slide. I
> tried picking up by starting at one side or end and letting tissue
> spread onto slide. Did not work.
> Also tried gently laying slide on top of sample and removing
> gently-still folds and air bubbles. Any help would be greatly
> appreciated as we have 6 more to do.
>
> Signed,
> Help!
> Terry Kokas
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 5
Date: Thu, 17 Nov 2011 14:58:19 -0500
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] RE: to cut or not to cut mouse brains
To: "McLaughlin, Terry " <tmclaugh <@t> NEMOURS.ORG>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C01C35B84DCDCE49BC60867E87F1C8FE3460069B54 <@t> USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"
Terry,
Here what works for us -
Slides are kept prechilled in a -20C freezer and then kept cold in the
cryostat.
Very gently press the cold slide onto the cut section so it makes contact
and the section sticks to the slide.
Flip slide over and use your finger to warm the back of the slide under the
section.
Starting with cold slides is a must for us.
Brett
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McLaughlin,
Terry
Sent: Thursday, November 17, 2011 2:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] to cut or not to cut mouse brains
Hello All,
I am having trouble cutting frozen mouse brains and was wondering if
someone can offer some help. The mouse was perfused in 4% PFA , the
brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
for 20 hrs, until the brain sank in this solution. It was frozen back by
using a culture plate floating in liquid nitrogen with OCT .
The problem I am having is many folds, and air bubbles under the tissue.
What I have done so far:
Cut at temp of -25, then tried - 22 and -17.
Cut at 10um, then tried 16-25um.
Tried making the knife colder by adding dry ice to it for a few seconds.
Added dry ice to sample for a couple seconds.
The sample cuts fine, it appears to happen when picking up on a slide. I
tried picking up by starting at one side or end and letting tissue
spread onto slide. Did not work.
Also tried gently laying slide on top of sample and removing
gently-still folds and air bubbles. Any help would be greatly
appreciated as we have 6 more to do.
Signed,
Help!
Terry Kokas
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
------------------------------
Message: 6
Date: Thu, 17 Nov 2011 15:00:37 -0500
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] RE: to cut or not to cut mouse brains
To: "McLaughlin, Terry " <tmclaugh <@t> NEMOURS.ORG>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C01C35B84DCDCE49BC60867E87F1C8FE3460069B5A <@t> USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"
Forgot to mention that we keep the cryostat around -16 to -18 C
Brett
-----Original Message-----
From: Connolly, Brett M
Sent: Thursday, November 17, 2011 2:58 PM
To: 'McLaughlin, Terry '; histonet <@t> lists.utsouthwestern.edu
Subject: RE: to cut or not to cut mouse brains
Terry,
Here what works for us -
Slides are kept prechilled in a -20C freezer and then kept cold in the
cryostat.
Very gently press the cold slide onto the cut section so it makes contact
and the section sticks to the slide.
Flip slide over and use your finger to warm the back of the slide under the
section.
Starting with cold slides is a must for us.
Brett
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McLaughlin,
Terry
Sent: Thursday, November 17, 2011 2:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] to cut or not to cut mouse brains
Hello All,
I am having trouble cutting frozen mouse brains and was wondering if
someone can offer some help. The mouse was perfused in 4% PFA , the
brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
for 20 hrs, until the brain sank in this solution. It was frozen back by
using a culture plate floating in liquid nitrogen with OCT .
The problem I am having is many folds, and air bubbles under the tissue.
What I have done so far:
Cut at temp of -25, then tried - 22 and -17.
Cut at 10um, then tried 16-25um.
Tried making the knife colder by adding dry ice to it for a few seconds.
Added dry ice to sample for a couple seconds.
The sample cuts fine, it appears to happen when picking up on a slide. I
tried picking up by starting at one side or end and letting tissue
spread onto slide. Did not work.
Also tried gently laying slide on top of sample and removing
gently-still folds and air bubbles. Any help would be greatly
appreciated as we have 6 more to do.
Signed,
Help!
Terry Kokas
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
------------------------------
Message: 7
Date: Thu, 17 Nov 2011 20:13:05 +0000
From: Helen Fedor <hfedor <@t> jhmi.edu>
Subject: RE: [Histonet] to cut or not to cut mouse brains
To: "'Grantham, Andrea L - (algranth)'" <algranth <@t> email.arizona.edu>,
"McLaughlin, Terry" <tmclaugh <@t> NEMOURS.ORG>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BCE07FB3A86FD040969A9EC0DD3EDA0D0B881C81 <@t> JHEMTMWEX4.win.ad.jhu.edu>
Content-Type: text/plain; charset="us-ascii"
When the blocks are not croyprotected you can cut them at warmer
temperatures. But when they are cryoprotected with the 30% sucrose, they are
soft at -18, that is why you need the colder temperature. they will compress
at the warmer temperatures and give you a lot of wrinkles.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Grantham,
Andrea L - (algranth)
Sent: Thursday, November 17, 2011 2:57 PM
To: McLaughlin, Terry
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] to cut or not to cut mouse brains
I'm interested in this answer because this has happened to me - but not
always.
I usually cut brains at warmer temps. like -17 or -18 as opposed to -25 and
they are usually handled in the same manner before being brought to the lab
frozen in OCT.
Andi
On Nov 17, 2011, at 12:24 PM, McLaughlin, Terry wrote:
> Hello All,
> I am having trouble cutting frozen mouse brains and was wondering if
> someone can offer some help. The mouse was perfused in 4% PFA , the
> brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
> for 20 hrs, until the brain sank in this solution. It was frozen back
> by using a culture plate floating in liquid nitrogen with OCT .
> The problem I am having is many folds, and air bubbles under the tissue.
> What I have done so far:
> Cut at temp of -25, then tried - 22 and -17.
> Cut at 10um, then tried 16-25um.
> Tried making the knife colder by adding dry ice to it for a few seconds.
> Added dry ice to sample for a couple seconds.
> The sample cuts fine, it appears to happen when picking up on a slide.
> I tried picking up by starting at one side or end and letting tissue
> spread onto slide. Did not work.
> Also tried gently laying slide on top of sample and removing
> gently-still folds and air bubbles. Any help would be greatly
> appreciated as we have 6 more to do.
>
> Signed,
> Help!
> Terry Kokas
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 8
Date: Thu, 17 Nov 2011 14:34:55 -0600
From: "Morocho, Jennifer" <Jmoroch1 <@t> Fairview.org>
Subject: [Histonet] HTL Lead Job Opening
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F0B374469354D64882E56C8F0B8DC6120134D08302 <@t> EXCH-MBX2.Fairview.org>
Content-Type: text/plain; charset="us-ascii"
The University of MN Medical Center, Fairview has an exciting and immediate
job opportunity as an HTL Technical Lead in Minneapolis, MN. Check out the
Fairview web site www.fairview.org<http://www.fairview.org> and look for job
11-36731. Or contact me, Jennifer Morocho, HTL (ASCP)CM at
jmoroch1 <@t> fairview.org<mailto:jmoroch1 <@t> fairview.org> for more details. The
Pathology Supervisor and I who would love to talk opportunity with you!
------------------------------
Message: 9
Date: Thu, 17 Nov 2011 15:37:03 -0500
From: "Goodwin, Diana" <dgoodwin <@t> rwjuhh.edu>
Subject: [Histonet] Brass cryostat chucks
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09411E0112A96A459D8D5FBDAB9C15C72480DAE2DF <@t> HAMEXMBA.rwjham.local>
Content-Type: text/plain; charset="us-ascii"
Greeting, Histonetters.
I am in search of those brass cryostat chucks with the holes in them. Any
ideas? Googled my brains out with no luck.
Diana G. Goodwin, BS, HT(ASCP)QIHC
Department of Pathology
Robert Wood Johnson University Hospital at Hamilton
One Hamilton Health Place
Hamilton, NJ 08690
Ph: 609.631.6996
Email: dgoodwin <@t> rwjuhh.edu
------------------------------
Message: 10
Date: Thu, 17 Nov 2011 16:33:49 -0500
From: "Ladd, Sharron" <Sharron.Ladd <@t> immunogen.com>
Subject: [Histonet] Source of IgG4 for Isotype control
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1EC9E4133CB4EB4A90383226A986461404CB34A93E <@t> wal-mail02>
Content-Type: text/plain; charset="us-ascii"
Hi Histonet,
Where can I buy IgG4 in the US please?
Thanks,
Sharron
------------------------------
Message: 11
Date: Thu, 17 Nov 2011 13:39:26 -0800
From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
Subject: Re: [Histonet] Brass cryostat chucks
To: "Goodwin, Diana" <dgoodwin <@t> rwjuhh.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C37ACD6A-80CB-44A6-8789-C2E9E33D7134 <@t> email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"
For what cryostat?
On Nov 17, 2011, at 1:37 PM, Goodwin, Diana wrote:
> Greeting, Histonetters.
>
> I am in search of those brass cryostat chucks with the holes in them. Any
ideas? Googled my brains out with no luck.
>
>
> Diana G. Goodwin, BS, HT(ASCP)QIHC
>
> Department of Pathology
>
> Robert Wood Johnson University Hospital at Hamilton
>
> One Hamilton Health Place
>
> Hamilton, NJ 08690
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Message: 12
Date: Thu, 17 Nov 2011 15:50:13 -0600
From: Christina.Wilson <@t> leica-microsystems.com
Subject: [Histonet] AUTO: is out of the office. (returning Wed
10/19/2011)
To: histonet <@t> lists.utsouthwestern.edu
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I am out of the office from Thu 11/17/2011 until Fri 11/18/2011.
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demaris.mills <@t> leica-microsystems.com, for product management support or
Karen Niewerth, karen.niewerth <@t> leica-microsystems.com, for customer service
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Note: This is an automated response to your message "Histonet Digest, Vol
96, Issue 26" sent on 11/17/2011 12:02:06 PM.
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Message: 13
Date: Thu, 17 Nov 2011 16:02:50 -0600
From: Salomao Segal <ssegal2 <@t> slu.edu>
Subject: [Histonet] microtomes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CACdnK8HuZek7dGaePt--kjzy219YnwgmEpiWq5DkoRPjtN8Aog <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
The settings in a rotary microtome may indicate the thickness of sections
but...
how do you know that it indeed cuts at the indicated thickness,
particularly if it is say an old device that you inherit from somebody
else's lab junk?
Is there a way of measuring the magnitude of advances after each rotation?
Thanks
Solomon Segal
------------------------------
Message: 14
Date: Thu, 17 Nov 2011 15:09:21 -0800 (PST)
From: Cheryl <tkngflght <@t> yahoo.com>
Subject: [Histonet] broken chuck holder
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1321571361.65679.YahooMailNeo <@t> web39409.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Is your tech using anything like ammonia or soap on her ice to soak the
blocks? This would chew through the spring much more quickly than just ice
water...???
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one GREAT Tech at a time.
281.852.9457 Office
800.756.3309 Phone & Fax
admin <@t> fullstaff.org
Sign up for the FREE newsletter AP News--updates, tricks of the trade and
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Message: 15
Date: Thu, 17 Nov 2011 15:46:16 -0800
From: connie grubaugh <conniegrubaugh <@t> hotmail.com>
Subject: RE: [Histonet] Paraplast X-tra
To: <one_angel_secret <@t> yahoo.com>, <cls71877 <@t> sbcglobal.net>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU153-W4295022D3E87DD9A19CF80D6C70 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
We are having problems again too. Have not changed a thing and convinced
that it is a bad lot again. Wonder sometimes on the QC that is done on the
paraffin.
Connie G.
> Date: Wed, 16 Nov 2011 13:47:28 -0800
> From: one_angel_secret <@t> yahoo.com
> To: cls71877 <@t> sbcglobal.net; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Paraplast X-tra
> CC:
>
> Maybe you got a bad lot that is contaminated causing the knife marks?
Also, Ive noticed that if you let your paraffin get to hot, it breaks down
and that could be one reason for a consistency issue. Have you changed
anything at all recently to your process? Hope this was helpful.
>
> Kim
>
>
> ________________________________
> From: Cristi stephenson <cls71877 <@t> sbcglobal.net>
> To: Histo Net <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, November 16, 2011 2:06 PM
> Subject: [Histonet] Paraplast X-tra
>
> Hello Histoland,
> We are seeing unusually large volumes of fall out in our paraffin lately.
> The precipitate is almost "muddy" or "oily" in appearance. We noticed it
> immediately as it was melting, so there was no way anything else had been
> introduced to cause this. As a result, we are seeing knife marks. The
marks
> are not consistent and not attributable to any of the tissue in the
blocks. We
> are blasting through blades that have been tried and true for years now.
Are
> there any other users of paraplast X-tra experiencing these issues?
>
> Thanks,
> Cristi
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Message: 16
Date: Thu, 17 Nov 2011 16:08:33 -0800
From: " cls71877 <@t> sbcglobal.net " <cls71877 <@t> sbcglobal.net>
Subject: Re: [Histonet] Paraplast X-tra
To: " connie grubaugh " <conniegrubaugh <@t> hotmail.com>,
one_angel_secret <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <74764.79442.bm <@t> smtp214.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=utf-8
I had to believe someone else out there was seeing this too!! Our rep
contacted us today and is going to replace it and investigate...I too wonder
how this gets past the QC dept., it is super obvious as soon as it starts
melting....
Sent from my HTC on the Now Network from Sprint!
----- Reply message -----
From: "connie grubaugh" <conniegrubaugh <@t> hotmail.com>
Date: Thu, Nov 17, 2011 3:46 pm
Subject: [Histonet] Paraplast X-tra
To: <one_angel_secret <@t> yahoo.com>, <cls71877 <@t> sbcglobal.net>,
<histonet <@t> lists.utsouthwestern.edu>
------------------------------
Message: 17
Date: Thu, 17 Nov 2011 20:32:04 -0700
From: "Karen Lahti" <karen <@t> gateslinger.com>
Subject: RE: [Histonet] RE: H. Pylori - IHC
To: "'Tom McNemar'" <TMcNemar <@t> lmhealth.org>,
<Beth.Fye <@t> HCAhealthcare.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <007701cca5a2$a4e88a90$eeb99fb0$@com>
Content-Type: text/plain; charset="us-ascii"
We use Biocare antibody with great results on the IntelliPath from Biocare
and the Bondmax from Leica.
Karen Lahti
Arizona Digestive Health
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, November 17, 2011 6:30 AM
To: 'Beth.Fye <@t> HCAhealthcare.com'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: H. Pylori - IHC
We get beautiful staining with Dako's concentrate on the Benchmark XT.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Beth.Fye <@t> HCAhealthcare.com
Sent: Wednesday, November 16, 2011 5:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H. Pylori - IHC
Does anyone have recommendations for a good vendor for H.Pylori for IHC?
We have tried many, but our pathologists complain that they are not specific
for H.Pylori and that all gram negative bacteria are staining. Currently we
are not running this but our pathologists are interested in it.
Thanks!
Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office: (804)228-6564
fax: (804)323-8638
<mailto:Beth.Fye <@t> hcahealthcare.com>
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