[Histonet] counterstain for fast red

Tony Henwood (SCHN) tony.henwood <@t> health.nsw.gov.au
Mon Nov 14 17:12:26 CST 2011

Try 1-2% Ethyl green in acetate buffer (pH4) - will give you green nuclei (of course)

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Tuesday, 15 November 2011 3:59 AM
To: Histonet
Subject: [Histonet] counterstain for fast red

Hi Everyone,
We are currently running some alk phos staining protocols with a fast red chromogen.  My pathologist would like to use something other than Hematoxylin as a counterstain.  What does everyone recommend? 

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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