[Histonet] Quiestion?

[SHANE NELSON]

I currently use a TBS for a GI lab and my temps for paraffin do not exceed 67 C during processing. I know it is recommended to be at 84 C, but at that temp it would fry everything and chatter was a serious issue even after soaking.
 

THANK YOU,
 
PATTI RUBEN-NELSON  H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonrnch <@t> verizon.net
 
 
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________________________________
From: "Willis, Donna G." <Donna.Willis <@t> baylorhealth.edu>
To: cheska mt <cheska91 <@t> hotmail.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 8, 2011 5:20 AM
Subject: RE: [Histonet] Quiestion?

The purpose of getting the paraffin up to 84C is to evaporate out the Isopropanol that is used instead of Xylene.  This does not mean that the paraffin should be kept at that temperature when it is not being used for the infiltration step.  It should be in an oven or a paraffin pot at a temperature no higher than 65C then allow the microwave to heat it up to 84C.  This also depends on the type of microwave that you are using.  If the unit has a vacuum system that is being used during the infiltration step then the temperature does not have reach 84C to evaporate out the IPA.  Read your user manual that from the manufacture, it should help.

Donna Willis, HT/HTL (ASCP)
Histology Lab Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465
donna.willis <@t> baylorhealth.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of cheska mt
Sent: Monday, November 07, 2011 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Quiestion?







From: cheska91 <@t> hotmail.com
To: histonet <@t> lists.utsouthwestern.edu
Subject: 
Date: Mon, 7 Nov 2011 11:32:09 -0800





Hello
    I am currently studying to become a histotech. I wanted to know why should the paraffin in the microwave processor be kept at 84 degrees.

Thanks
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