[Histonet] microwave processing

[Tony Henwood (SCHN)]

I believe that the higher temperature is needed to evaporate off the isopropanol.
Just make sure that your tissue is well fixed.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Zoe rosa
Sent: Monday, 7 November 2011 1:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] microwave processing


Hi there,
 
On an Instrumentation course at keiser, we had a discussion about the right temperature fro the paraffin when the proceesing is done using microwaves. Can some tell me what should be the ideal temperature with out damaging the processing. The text book mentions 84 degrees, but isn't this to high; perhaps if the time is less, it wont affect it?
 
Thanks,
 
Ismael Lopez 		 	   		  _______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
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