[Histonet] problem with brain sections falling off subbed slides

King,Michael A making <@t> ufl.edu
Thu Nov 3 08:04:25 CDT 2011


Apart from Dr. Kiernan's recommendation, you can try putting your 
mounted slides in fixative for a few minutes, we've found this to work 
with gelatin-only coated slides, or chrome-alum gelatin subbed slides 
with thick brain sections.  If you're trying to rescue material already 
on slides, dip briefly in warm 1% gelatin, air dry, fix for a few 
minutes in 10% NBF, and proceed to counterstaining.

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Message: 14
Date: Wed, 2 Nov 2011 17:06:48 -0500
 From: Douglas M Burns <dmburns9 <@t> gmail.com>
Subject: [Histonet] problem with brain sections falling off subbed
	slides
Hello,
       I am new to working with brain, and I am having a bad problem 
with
both thin and thick rat brain sections sliding off of subbed slides...




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