[Histonet] RE: Luciferase IHC!

[Joe Nocito]

I'm here.  I was asking my Priest the steps to perform an exorcism, although 
the Catholic Church doesn't perform these rituals any longer. I have all the 
materials, readings and "exorcism paraphernalia" Just let me know where and 
when. Just make sure there is a good bottle of Scotch available, just in 
case all else fails.

JTT
----- Original Message ----- 
From: "Marsh, Nannette" <NMP <@t> stowers.org>
To: "'Randolph-Habecker, Julie'" <jhabecke <@t> fhcrc.org>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, November 02, 2011 11:31 AM
Subject: [Histonet] RE: Luciferase IHC!


This is great.  I've enjoyed the story line and witty comments and remarks. 
Thanks,Nanne

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
Randolph-Habecker, Julie
Sent: Wednesday, November 02, 2011 10:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Luciferase IHC!

Thanks for the help!
We actually tried antigen retrieval in Holy water in a pressure cooker for 
10 minutes. I even brought in an old priest and a young priest to perform 
the staining but no luck! Sorry, no vendor for the holy water - the old 
priest makes his own.

I enjoyed the laugh! This project has been hell!

Thanks,

Julie

Message: 21
Date: Wed, 2 Nov 2011 08:46:28 -0500
From: "Beckham, Sharon" <SLB <@t> stowers.org>
Subject: RE: [Histonet] Luciferase IHC
To: "'O'Donnell, Bill'" <billodonnell <@t> catholichealth.net>, Kim Donadio
<one_angel_secret <@t> yahoo.com>, "Randolph-Habecker, Julie"
<jhabecke <@t> fhcrc.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C40E43D1F7A56408C4463FD245DDDF98AEE2C53 <@t> EXCHMB-02.stowers-institute.org>

Content-Type: text/plain; charset="iso-8859-1"

You know sometimes I wish these emails had a "LIKE" button on them.   I 
found this quite funny and wanted to "LIKE" it.  Too much facebook for me!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, 
Bill
Sent: Wednesday, November 02, 2011 8:19 AM
To: Kim Donadio; Randolph-Habecker, Julie; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Luciferase IHC

Actually, I have found that holy water reduces its sensitivity, greatly 
diminishes its signal and will destroy its reactivity. (Listen closely and 
you will hear the slide scream and growl and tell you no one likes you) 
(It's only Wednesday, what'll Friday look like?)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, November 01, 2011 7:01 PM
To: Randolph-Habecker, Julie; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC

You have to soak it in holy water, then all H311 will break out,,,,

Sorry, I couldnt resist.




________________________________
From: "Randolph-Habecker, Julie" <jhabecke <@t> fhcrc.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, November 1, 2011 6:49 PM
Subject: [Histonet] Luciferase IHC

Has anyone had good results staining for luciferase in FFPE tissue? If so, 
what antibody did you use.



Thanks!!



Julie



Julie Randolph-Habecker, Ph.D.

Director, Experimental Histopathology Shared Resources

Fred Hutchinson Cancer Research Center

1100 Fairview Ave N, DE-360

Seattle WA 98109-1024

Tel: 206-667-6119

Fax: 206-667-6845

jhabecke <@t> fhcrc.org



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End of Histonet Digest, Vol 96, Issue 3
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