[Histonet] Luciferase IHC!

[Randolph-Habecker, Julie]

Thanks for the help!
We actually tried antigen retrieval in Holy water in a pressure cooker for 10 minutes. I even brought in an old priest and a young priest to perform the staining but no luck! Sorry, no vendor for the holy water - the old priest makes his own.

I enjoyed the laugh! This project has been hell!

Thanks,

Julie

Message: 21
Date: Wed, 2 Nov 2011 08:46:28 -0500
From: "Beckham, Sharon" <SLB <@t> stowers.org>
Subject: RE: [Histonet] Luciferase IHC
To: "'O'Donnell, Bill'" <billodonnell <@t> catholichealth.net>, Kim Donadio
	<one_angel_secret <@t> yahoo.com>, "Randolph-Habecker, Julie"
	<jhabecke <@t> fhcrc.org>, "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<2C40E43D1F7A56408C4463FD245DDDF98AEE2C53 <@t> EXCHMB-02.stowers-institute.org>
	
Content-Type: text/plain; charset="iso-8859-1"

You know sometimes I wish these emails had a "LIKE" button on them.   I found this quite funny and wanted to "LIKE" it.  Too much facebook for me! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Wednesday, November 02, 2011 8:19 AM
To: Kim Donadio; Randolph-Habecker, Julie; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Luciferase IHC

Actually, I have found that holy water reduces its sensitivity, greatly diminishes its signal and will destroy its reactivity. (Listen closely and you will hear the slide scream and growl and tell you no one likes you) (It's only Wednesday, what'll Friday look like?)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, November 01, 2011 7:01 PM
To: Randolph-Habecker, Julie; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC

You have to soak it in holy water, then all H311 will break out,,,,
 
Sorry, I couldnt resist. 
 
 


________________________________
From: "Randolph-Habecker, Julie" <jhabecke <@t> fhcrc.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, November 1, 2011 6:49 PM
Subject: [Histonet] Luciferase IHC

Has anyone had good results staining for luciferase in FFPE tissue? If so, what antibody did you use.



Thanks!!



Julie



Julie Randolph-Habecker, Ph.D.

Director, Experimental Histopathology Shared Resources

Fred Hutchinson Cancer Research Center

1100 Fairview Ave N, DE-360

Seattle WA 98109-1024

Tel: 206-667-6119

Fax: 206-667-6845

jhabecke <@t> fhcrc.org



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End of Histonet Digest, Vol 96, Issue 3
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