[Histonet] RE: Luciferase IHC

[Connolly, Brett M]

Julie, 
Are you looking to stain for firefly luciferase to confirm localization of an optical imaging probe?

That's what I want to do, there are antibodies available from Lifespan and Biovision among others that are purported to work on FFPE sections ....I have yet to try them.

You can check out this article, but they used a UK supplier. 

Bioluminescence imaging to monitor bladder cancer cell adhesion in vivo: a new approach to optimize a syngeneic, orthotopic, murine bladder cancer model.

Jurczok A, Fornara P, Söling A.

BJU Int. 2008 Jan;101(1):120-4. Epub 2007 Sep 20.

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803




. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie
Sent: Tuesday, November 01, 2011 6:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Luciferase IHC

Has anyone had good results staining for luciferase in FFPE tissue? If
so, what antibody did you use.

 

Thanks!!

 

Julie

 

Julie Randolph-Habecker, Ph.D.

Director, Experimental Histopathology Shared Resources

Fred Hutchinson Cancer Research Center

1100 Fairview Ave N, DE-360

Seattle WA 98109-1024

Tel: 206-667-6119

Fax: 206-667-6845

jhabecke <@t> fhcrc.org

 

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