[Histonet] RE: mast cells for arteries

[C.M. van der Loos]

Patsy,
Just consider anti-tryptase antibody that is directly labeled with Alk. phos. (Millipore MAB1222A). Tris-EDTA pH9.0 pretreatment / 1:5000 overnight 4C / Vector Red. A tonsil will do as positive control.
Good luck!
Cheers, Chris

Chris van der Loos
Academic Medical Center
Dept. of Pathology
Amsterdam 
The Netherlands



Date: Tue, 1 Nov 2011 11:07:47 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] mast cells for arteries
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4937809CEF224CCE99B75C4F01EC4D5E <@t> Patsyoffice>
Content-Type: text/plain;	charset="us-ascii"

Does anyone have a go to stain for Mast Cells besides Tol Blue?  We are trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining, we used NH Skin and got maybe a few mast cells but I would like to use something more abundant with mast cells.

 

Best regards,

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010