[Histonet] RE: mast cells for arteries

C.M. van der Loos c.m.vanderloos <@t> amc.uva.nl
Wed Nov 2 08:58:38 CDT 2011

Just consider anti-tryptase antibody that is directly labeled with Alk. phos. (Millipore MAB1222A). Tris-EDTA pH9.0 pretreatment / 1:5000 overnight 4C / Vector Red. A tonsil will do as positive control.
Good luck!
Cheers, Chris

Chris van der Loos
Academic Medical Center
Dept. of Pathology
The Netherlands

Date: Tue, 1 Nov 2011 11:07:47 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] mast cells for arteries
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4937809CEF224CCE99B75C4F01EC4D5E <@t> Patsyoffice>
Content-Type: text/plain;	charset="us-ascii"

Does anyone have a go to stain for Mast Cells besides Tol Blue?  We are trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining, we used NH Skin and got maybe a few mast cells but I would like to use something more abundant with mast cells.


Best regards,



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010

More information about the Histonet mailing list